|
| Status |
Public on Dec 06, 2016 |
| Title |
normal tongue, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Mus musculus, control group
|
| Organism |
Mus musculus |
| Characteristics |
tissue: tongue
genotype: CBA
gender: male
age (weeks): 24
tongue section: normal
|
| Treatment protocol |
Immediately following death, tongue were excised, longitudinally bisected, each half of the tongue was frozen on dry ice, embedded in OCT and stored at -80°C. Epithelial cells were isolated using an infrared laser microdissection system (Arcturus PixCell II®, Life Technologies, NY, USA). Microdissected cells on the cap were solubilized using a RLT lysis buffer and were subjected to total RNA extraction.
|
| Growth protocol |
Three mice were from the control group and 9 mice were from the group treated by the 4-NQO.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was performed using the Rneasy Microkit® (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Following amplification of 2ng of RNA (Ovation Pico WTA System v2, NuGEN Technologies), each amplified sense cDNA product was fragmented and biotinylated, using an Encore Biotin Module according to manufacturer’s instructions (NuGEN Technologies).
|
| |
|
| Hybridization protocol |
Each sample was hybridized onto a GeneChip Mouse Gene ST 2.0 Array (Affymetrix,Santa Clara, CA, USA)
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 (Affymetrix)
|
| Description |
Gene expression data from microdissected cells in murine tongue,normal
|
| Data processing |
The data were analyzed with the software Array Studio (Omicsoft Corporation) using quantile normalization and RMA method.
|
| |
|
| Submission date |
Nov 25, 2015 |
| Last update date |
Dec 06, 2016 |
| Contact name |
PIERRE SAINTIGNY |
| E-mail(s) |
PIERRE.SAINTIGNY@LYON.UNICANCER.FR
|
| Organization name |
Centre Léon Bérard
|
| Street address |
28 Rue Laennec
|
| City |
Lyon |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL18802 |
| Series (1) |
| GSE75421 |
Expression data of tongue mucosa from normal mice and mice treated by the 4-NQO |
|
