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Sample GSM1955361 Query DataSets for GSM1955361
Status Public on Jun 07, 2016
Title PGC-1a2 2
Sample type RNA
 
Source name PGC-1a isoform 2
Organism Mus musculus
Characteristics cell type: Mouse primary myoblasts
Treatment protocol Upon myotube formation (48h after induction of differentiation) we expressed GFP alone or GFP and one of the four PGC-1α isoforms via adenoviral vectors at an MOI of 100. GFP and PGC-1α isoforms are expressed from independent promoters. Adenoviruses expressing the different mouse PGC1α isoforms or GFP have been previously described (3). Twelve hours after transduction, media was replaced with fresh Differentiation Media. Approximately 60 hours post-transduction, cells were harvested in Isol-RNA lysis reagent.
Growth protocol During the growth phase, mouse primary myoblasts were plated in collagen-coated plates and maintained in Ham´s F-10 Nutrient Mixture (Gibco), 20% FBS (Sigma), 1% Penicillin/Streptomycin (Gibco) and 2.5 ng/ml basic Fibroblast Growth Factor (Gibco). After achieving 90% confluence, cells were changed to Differentiation Media consisting of DMEM (Gibco), 5% Horse Serum (Gibco), and 1% Penicillin/Streptomycin.
Extracted molecule total RNA
Extraction protocol Approximately 60 hours post-transduction, cells were harvested in Isol-RNA lysis reagent (5PRIME) and purified with Nucleospin RNA II columns (Macherey-Nagel) according to manufacturer’s instructions to achieve optimal purity levels
Label biotin
Label protocol Total RNA was converted to sense strand cDNA using Ambion WT Expression kit. For fragmentation and labeling WT GeneChip WT target Labeling kit was used.
 
Hybridization protocol According to Affymetrix protocol
Scan protocol According to Affymetrix protocol
Data processing Data were processed through Qlucore and Partek.
 
Submission date Nov 27, 2015
Last update date Jun 07, 2016
Contact name Karin Dahlman-Wright
Organization name Karolinska Institutet
Department Department of Biosciences and Nutrition
Lab Medicinaren 25/Neo
Street address Blickagången 16
City Huddinge
ZIP/Postal code 14157
Country Sweden
 
Platform ID GPL6096
Series (1)
GSE75448 Peroxisome proliferator-activated receptor gamma coactivator-1alpha isoforms selectively regulate multiple splicing events on target genes.

Data table header descriptions
ID_REF
VALUE RMA normalized log2 values

Data table
ID_REF VALUE
4305140 3.8988
4305147 9.51041
4305425 5.64842
4305730 6.44193
4305790 4.35076
4305808 4.50791
4305936 4.72925
4306126 2.08658
4306193 4.38165
4306220 8.58714
4306323 4.59878
4306467 5.26887
4306475 5.22188
4306482 2.22767
4306490 10.8972
4306955 10.4105
4307112 4.60282
4307259 7.02467
4307291 8.71726
4307433 3.19181

Total number of rows: 23332

Table truncated, full table size 362 Kbytes.




Supplementary file Size Download File type/resource
GSM1955361_HG08_A2.2_MoEx-1_0-st-v1_.CEL.gz 21.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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