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Sample GSM1955363 Query DataSets for GSM1955363
Status Public on Jun 07, 2016
Title PGC-1a3 1
Sample type RNA
 
Source name PGC-1a isoform 3
Organism Mus musculus
Characteristics cell type: Mouse primary myoblasts
Treatment protocol Upon myotube formation (48h after induction of differentiation) we expressed GFP alone or GFP and one of the four PGC-1α isoforms via adenoviral vectors at an MOI of 100. GFP and PGC-1α isoforms are expressed from independent promoters. Adenoviruses expressing the different mouse PGC1α isoforms or GFP have been previously described (3). Twelve hours after transduction, media was replaced with fresh Differentiation Media. Approximately 60 hours post-transduction, cells were harvested in Isol-RNA lysis reagent.
Growth protocol During the growth phase, mouse primary myoblasts were plated in collagen-coated plates and maintained in Ham´s F-10 Nutrient Mixture (Gibco), 20% FBS (Sigma), 1% Penicillin/Streptomycin (Gibco) and 2.5 ng/ml basic Fibroblast Growth Factor (Gibco). After achieving 90% confluence, cells were changed to Differentiation Media consisting of DMEM (Gibco), 5% Horse Serum (Gibco), and 1% Penicillin/Streptomycin.
Extracted molecule total RNA
Extraction protocol Approximately 60 hours post-transduction, cells were harvested in Isol-RNA lysis reagent (5PRIME) and purified with Nucleospin RNA II columns (Macherey-Nagel) according to manufacturer’s instructions to achieve optimal purity levels
Label biotin
Label protocol Total RNA was converted to sense strand cDNA using Ambion WT Expression kit. For fragmentation and labeling WT GeneChip WT target Labeling kit was used.
 
Hybridization protocol According to Affymetrix protocol
Scan protocol According to Affymetrix protocol
Data processing Data were processed through Qlucore and Partek.
 
Submission date Nov 27, 2015
Last update date Jun 07, 2016
Contact name Karin Dahlman-Wright
Organization name Karolinska Institutet
Department Department of Biosciences and Nutrition
Lab Medicinaren 25/Neo
Street address Blickagången 16
City Huddinge
ZIP/Postal code 14157
Country Sweden
 
Platform ID GPL6096
Series (1)
GSE75448 Peroxisome proliferator-activated receptor gamma coactivator-1alpha isoforms selectively regulate multiple splicing events on target genes.

Data table header descriptions
ID_REF
VALUE RMA normalized log2 values

Data table
ID_REF VALUE
4305140 3.69822
4305147 9.65005
4305425 4.68404
4305730 6.1567
4305790 4.53958
4305808 3.97451
4305936 4.31966
4306126 2.87507
4306193 3.54713
4306220 8.72057
4306323 4.45087
4306467 4.777
4306475 5.0314
4306482 1.66458
4306490 11.1121
4306955 10.5986
4307112 4.68268
4307259 7.26082
4307291 8.50275
4307433 3.09747

Total number of rows: 23332

Table truncated, full table size 362 Kbytes.




Supplementary file Size Download File type/resource
GSM1955363_HG10_A3.1_MoEx-1_0-st-v1_.CEL.gz 21.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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