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Sample GSM1955367 Query DataSets for GSM1955367
Status Public on Jun 07, 2016
Title PGC-1a4 2
Sample type RNA
 
Source name PGC-1a isoform 1
Organism Mus musculus
Characteristics cell type: Mouse primary myoblasts
Treatment protocol Upon myotube formation (48h after induction of differentiation) we expressed GFP alone or GFP and one of the four PGC-1α isoforms via adenoviral vectors at an MOI of 100. GFP and PGC-1α isoforms are expressed from independent promoters. Adenoviruses expressing the different mouse PGC1α isoforms or GFP have been previously described (3). Twelve hours after transduction, media was replaced with fresh Differentiation Media. Approximately 60 hours post-transduction, cells were harvested in Isol-RNA lysis reagent.
Growth protocol During the growth phase, mouse primary myoblasts were plated in collagen-coated plates and maintained in Ham´s F-10 Nutrient Mixture (Gibco), 20% FBS (Sigma), 1% Penicillin/Streptomycin (Gibco) and 2.5 ng/ml basic Fibroblast Growth Factor (Gibco). After achieving 90% confluence, cells were changed to Differentiation Media consisting of DMEM (Gibco), 5% Horse Serum (Gibco), and 1% Penicillin/Streptomycin.
Extracted molecule total RNA
Extraction protocol Approximately 60 hours post-transduction, cells were harvested in Isol-RNA lysis reagent (5PRIME) and purified with Nucleospin RNA II columns (Macherey-Nagel) according to manufacturer’s instructions to achieve optimal purity levels
Label biotin
Label protocol Total RNA was converted to sense strand cDNA using Ambion WT Expression kit. For fragmentation and labeling WT GeneChip WT target Labeling kit was used.
 
Hybridization protocol According to Affymetrix protocol
Scan protocol According to Affymetrix protocol
Data processing Data were processed through Qlucore and Partek.
 
Submission date Nov 27, 2015
Last update date Jun 07, 2016
Contact name Karin Dahlman-Wright
Organization name Karolinska Institutet
Department Department of Biosciences and Nutrition
Lab Medicinaren 25/Neo
Street address Blickagången 16
City Huddinge
ZIP/Postal code 14157
Country Sweden
 
Platform ID GPL6096
Series (1)
GSE75448 Peroxisome proliferator-activated receptor gamma coactivator-1alpha isoforms selectively regulate multiple splicing events on target genes.

Data table header descriptions
ID_REF
VALUE RMA normalized log2 values

Data table
ID_REF VALUE
4305140 4.0093
4305147 9.90386
4305425 5.77883
4305730 5.6538
4305790 5.17542
4305808 3.69501
4305936 2.88986
4306126 2.71648
4306193 3.4274
4306220 8.60975
4306323 3.43458
4306467 5.47014
4306475 4.55209
4306482 1.78166
4306490 11.03
4306955 10.7539
4307112 4.91374
4307259 7.69245
4307291 9.08901
4307433 2.75612

Total number of rows: 23332

Table truncated, full table size 361 Kbytes.




Supplementary file Size Download File type/resource
GSM1955367_HG14_A4.2_MoEx-1_0-st-v1_.CEL.gz 22.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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