|
| Status |
Public on Mar 31, 2016 |
| Title |
hindlimb muscle of aged mice, ZT 6, repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
hindlimb muscle of aged_ZT 6
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
zt: 6
age (weeks): 80
tissue: hindlimb muscle
repeat: 2
|
| Treatment protocol |
Beginning with ZT 0 and afterwards in intervals of 3 hours, 3 young and 3 aged mice where sacrificed. Hindlimb muscles where extracted, shock frozen in liquid nitrogen and stored at 193 K. Sample collection was done at 16 different time points over 2 sucsessive days.
|
| Growth protocol |
Mice of two different age groups (young = 8 weeks; aged = 80 weeks) where kept under 12 h light / 12 h dark conditions for 2 weeks to ensure stable circadian entrainment. Food and water where supplied ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Muscle samples where homogenized using the gentleMacs system following the manufacturer's instructions for frozen tissue. Extraction of total RNA was made with Trizol following manufacturers instructions. Purified RNA was redissolved in RNAse free water.
|
| Label |
biotin
|
| Label protocol |
RNA samples from 3 different animals sampled at the same time point were pooled and labeled as recommended by the manufacturer with the labeling kit for GeneChip Mouse Gene 2.0 ST Arrays.
|
| |
|
| Hybridization protocol |
Labeled RNA was loaded on GeneChip Mouse Gene 2.0 ST Array chips following the manufacturer's instructions.
|
| Scan protocol |
Chips where scanned in Affymetrix GCS3000 high resolution scanner.
|
| Description |
Gene expression in aged mouse muscle at ZT 6
Mmu_Muscle_aged_ZT06_2
|
| Data processing |
Data where processed with R (v3.2.2) using the package affy (v1.44.1). For normalization and summarization, the RMA procedure with custom made CDFs from the Brainarray project (mogene20st_Mm_ENTREZG; v18) were used.
|
| |
|
| Submission date |
Nov 30, 2015 |
| Last update date |
Mar 31, 2016 |
| Contact name |
Paul Florian Thaben |
| E-mail(s) |
paul.thaben@charite.de
|
| Organization name |
Charite Universitätsmedizin Berlin
|
| Department |
Institute for Theoretical Biology
|
| Lab |
AG Westermark
|
| Street address |
Philippstr. 13, Haus 4
|
| City |
Berlin |
| State/province |
Berlin |
| ZIP/Postal code |
10115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18802 |
| Series (1) |
| GSE75523 |
Circadian mRNA expression in skeletal muscle of young and aged mice |
|
