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Sample GSM1964812 Query DataSets for GSM1964812
Status Public on Feb 01, 2016
Title BT474_1day_vehicle_rep1
Sample type RNA
 
Source name BT474 cells treated for 24h with vehicle
Organism Homo sapiens
Characteristics cell line: breast cancer cell line BT474
agent: control
Treatment protocol Reagents were simply added to the media for cell treatment in regular 6cm culture dish.
Growth protocol Cells were cultured in regular DMEM. 24h after seeding, media was changed and fresh media with vehicle or AICAR 0.5mM
Extracted molecule total RNA
Extraction protocol After 24h with vehicle or AICAR, RNA was purified with Aurum Total RNA Mini-Kit (Bio-Rad).
Label biotin
Label protocol Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
 
Hybridization protocol RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Data processing affymetrix-algorithm-name = rma
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.3.1.187
The expression data were analyzed using FlexArray10 software.
 
Submission date Dec 05, 2015
Last update date Feb 02, 2016
Contact name Etienne Audet-Walsh
E-mail(s) etienne.audet-walsh@crchudequebec.ulaval.ca
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL570
Series (2)
GSE75727 The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer [Microarray expression]
GSE75877 The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 9.48419
AFFX-BioB-M_at 9.44456
AFFX-BioB-3_at 9.33676
AFFX-BioC-5_at 10.2776
AFFX-BioC-3_at 11.0881
AFFX-BioDn-5_at 11.8656
AFFX-BioDn-3_at 13.0588
AFFX-CreX-5_at 13.6833
AFFX-CreX-3_at 14.0216
AFFX-DapX-5_at 8.52263
AFFX-DapX-M_at 9.97889
AFFX-DapX-3_at 10.5032
AFFX-LysX-5_at 6.51938
AFFX-LysX-M_at 6.76367
AFFX-LysX-3_at 7.47582
AFFX-PheX-5_at 7.14862
AFFX-PheX-M_at 7.50015
AFFX-PheX-3_at 8.07671
AFFX-ThrX-5_at 7.34446
AFFX-ThrX-M_at 7.52679

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM1964812_1_Day_Control_1_JSP013-2_01.CEL.gz 4.8 Mb (ftp)(http) CEL
GSM1964812_1_Day_Control_1_JSP013-2_01.rma.chp.gz 376.9 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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