|
| Status |
Public on Feb 01, 2016 |
| Title |
BT474_1day_vehicle_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
BT474 cells treated for 24h with vehicle
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: breast cancer cell line BT474
agent: control
|
| Treatment protocol |
Reagents were simply added to the media for cell treatment in regular 6cm culture dish.
|
| Growth protocol |
Cells were cultured in regular DMEM. 24h after seeding, media was changed and fresh media with vehicle or AICAR 0.5mM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 24h with vehicle or AICAR, RNA was purified with Aurum Total RNA Mini-Kit (Bio-Rad).
|
| Label |
biotin
|
| Label protocol |
Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
|
| |
|
| Hybridization protocol |
RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
|
| Scan protocol |
Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
|
| Data processing |
affymetrix-algorithm-name = rma
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.3.1.187
The expression data were analyzed using FlexArray10 software.
|
| |
|
| Submission date |
Dec 05, 2015 |
| Last update date |
Feb 02, 2016 |
| Contact name |
Etienne Audet-Walsh |
| E-mail(s) |
etienne.audet-walsh@crchudequebec.ulaval.ca
|
| Organization name |
Université Laval
|
| Department |
Molecular Medicine
|
| Street address |
2705 boulevard Laurier
|
| City |
Québec City |
| State/province |
QC |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE75727 |
The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer [Microarray expression] |
| GSE75877 |
The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer |
|
