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Sample GSM1964815 Query DataSets for GSM1964815
Status Public on Feb 01, 2016
Title BT474_1day_AICAR_rep2
Sample type RNA
 
Source name BT474 cells treated for 24h with AICAR
Organism Homo sapiens
Characteristics cell line: breast cancer cell line BT474
agent: AICAR
Treatment protocol Reagents were simply added to the media for cell treatment in regular 6cm culture dish.
Growth protocol Cells were cultured in regular DMEM. 24h after seeding, media was changed and fresh media with vehicle or AICAR 0.5mM
Extracted molecule total RNA
Extraction protocol After 24h with vehicle or AICAR, RNA was purified with Aurum Total RNA Mini-Kit (Bio-Rad).
Label biotin
Label protocol Labeling was performed using standard Affymetrix protocol at Génome Québec Innovation Centre (McGill University)
 
Hybridization protocol RNA was hybridized to GeneChip® Human Genome U133 Plus 2.0 (Affymetrix) and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Data processing affymetrix-algorithm-name = rma
affymetrix-algorithm-version = 1.0
program-name = Expression Console
program-version = 1.3.1.187
The expression data were analyzed using FlexArray10 software.
 
Submission date Dec 05, 2015
Last update date Feb 02, 2016
Contact name Etienne Audet-Walsh
E-mail(s) etienne.audet-walsh@crchudequebec.ulaval.ca
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
 
Platform ID GPL570
Series (2)
GSE75727 The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer [Microarray expression]
GSE75877 The PGC-1α/ERRα Axis Represses One-Carbon Metabolism and Promotes Sensitivity to Anti-Folate Therapy in Breast Cancer

Data table header descriptions
ID_REF
VALUE RMA signal

Data table
ID_REF VALUE
AFFX-BioB-5_at 9.45167
AFFX-BioB-M_at 9.47944
AFFX-BioB-3_at 9.27236
AFFX-BioC-5_at 10.3049
AFFX-BioC-3_at 11.0495
AFFX-BioDn-5_at 11.9132
AFFX-BioDn-3_at 13.0974
AFFX-CreX-5_at 13.7183
AFFX-CreX-3_at 14.1355
AFFX-DapX-5_at 8.3392
AFFX-DapX-M_at 9.90579
AFFX-DapX-3_at 10.5814
AFFX-LysX-5_at 6.41521
AFFX-LysX-M_at 6.62362
AFFX-LysX-3_at 7.32931
AFFX-PheX-5_at 7.02723
AFFX-PheX-M_at 7.46427
AFFX-PheX-3_at 7.9971
AFFX-ThrX-5_at 7.02747
AFFX-ThrX-M_at 7.43898

Total number of rows: 54675

Table truncated, full table size 998 Kbytes.




Supplementary file Size Download File type/resource
GSM1964815_1_Day_AICAR_2_JSP016-2_01.CEL.gz 5.0 Mb (ftp)(http) CEL
GSM1964815_1_Day_AICAR_2_JSP016-2_01.rma.chp.gz 377.1 Kb (ftp)(http) CHP
Raw data provided as supplementary file
Processed data included within Sample table
Processed data provided as supplementary file

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