|
| Status |
Public on Jun 01, 2016 |
| Title |
G3_IB_ENEG_GPOS_1 |
| Sample type |
RNA |
| |
|
| Source name |
Endometrium cancer, FPPE section
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: Endometrium cancer
grade: 3
figo stage: 1B
lymphovascular space invasion status: LVSI-
lymph node status: Nodal +
sample origin: FPPE section
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNeasy FFPE Kit (Qiagen, Courtaboeuf, France), according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
After validation of the RNA quality with Bioanalyzer 2100, 1 µg of total RNA was biotin labelled following the FlashTag Biotin HSR RNA labelling kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, mirRNA targets were hybridized for 20 hr at 48C on mirRNA-40 Affymetrix. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Description |
G3_IB_NEG_POS
|
| Data processing |
The data were preprocessed and analyzing using Partek Genomic Suite.
|
| |
|
| Submission date |
Dec 14, 2015 |
| Last update date |
Jun 01, 2016 |
| Contact name |
Geoffroy - CANLORBE |
| E-mail(s) |
geoffroy.canlorbe@aphp.fr
|
| Phone |
+33 1 56 01 60 19
|
| Organization name |
Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), University Pierre and Marie Curie, Paris 6
|
| Department |
Department of Obstetrics and Gynaecology
|
| Lab |
-
|
| Street address |
4, rue de la Chine
|
| City |
Paris |
| State/province |
- |
| ZIP/Postal code |
75020 |
| Country |
France |
| |
|
| Platform ID |
GPL19117 |
| Series (1) |
| GSE75968 |
microRNA expression profile related to lymph node status in women with stage grade 1-2-3 endometrial cancer |
|
