|
| Status |
Public on Jul 01, 2007 |
| Title |
striatum_Q92_18m_11 |
| Sample type |
RNA |
| |
|
| Source name |
Striatum (brain)
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6; Gender: male; Age: 596 days; Tissue: striatum (brain); Mouse model: HdhQ92/Q92 knock-in mice carrying 92 CAG repeats derived from exon 1 of human HD gene knocked in to of the endogenous mouse gene (Wheeler, V.C. et al. Length-dependent gametic CAG repeat instability in the Huntington's disease knock-in mouse. Hum. Mol. Genet. 8, 115-122 (1999))
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol (Invitrogen) followed by RNeasy column cleanup (Qiagen) using the manufacturers’ protocols
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip® IVT Labeling Kit, according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| |
|
| Hybridization protocol |
Affymetrix Eukaryotic Target Hybridization protocol for 100 Format midi arrays (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| Scan protocol |
Affymetrix® GeneChip® Scanner 3000 with GCOS software according to manufactuer's protocol (GeneChip® Expression Analysis Technical Manual 701021 Rev. 5)
|
| Description |
Affymetrix GeneChip® Mouse Genome 430 2.0 Array
|
| Data processing |
Probe set summarization and normalization was performed by robust multi-array averaging (RMA, including background subtraction, summarization by median polish and quantile normalization) using the R/Bioconductor package affy.
|
| |
|
| Submission date |
May 31, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Alexandre Kuhn |
| E-mail(s) |
kuhnam@mail.nih.gov
|
| Organization name |
NIH
|
| Department |
NIA
|
| Lab |
Laboratory of Neurogenetics
|
| Street address |
35 Convent Dr
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892-3707 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE7958 |
Striatal gene expression data from 3- and 18-month-old Q92 mice and control mice. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
