|
| Status |
Public on Aug 14, 2007 |
| Title |
G9a-WT |
| Sample type |
RNA |
| |
|
| Source name |
Mouse Embryonic fibroblast
|
| Organism |
Mus musculus |
| Characteristics |
G9a-deleted MEFs reconstituted with wild type FLAG-G9a (WT)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol /RNAeasy cleaning
|
| Label |
Biotin
|
| Label protocol |
Affymetrix standard one round RT-IVT labeling.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Affymetrix GS3000 scanner - 5um scanning
|
| Description |
Transcription profiling involves H3 methyltransferase G9a-deleted MEFs reconstituted with empty vector (delta), wild type FLAG-G9a (WT), FLAG-G9a K165A (K165A) or FLAG-G9a H1093K catalytic mutant (H1093K).
|
| Data processing |
OVerall intensity of the image was targeted to 500
Probe set intensity was extracted by GCOS 1.4
|
| |
|
| Submission date |
Jun 04, 2007 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Agnes Viale |
| E-mail(s) |
vialea@mskcc.org
|
| Phone |
212 639 3031
|
| Fax |
212 717 3053
|
| Organization name |
Memorial Sloan Kettering Cancer Center
|
| Department |
Molecular Biology
|
| Lab |
Genomics Core Laboratory
|
| Street address |
1275 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE8011 |
Methylation of hMTase G9a on a histone-like site regulates protein complex assembly |
|
