NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM197749 Query DataSets for GSM197749
Status Public on Aug 14, 2007
Title G9a-deleted
Sample type RNA
 
Source name mouse embryonic fibroblast
Organism Mus musculus
Characteristics G9a-deleted MEFs
Extracted molecule total RNA
Extraction protocol Trizol /RNAeasy cleaning
Label Biotin
Label protocol Affymetrix standard one round RT-IVT labeling.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Affymetrix GS3000 scanner - 5um scanning
Description Transcription profiling involves H3 methyltransferase G9a-deleted MEFs reconstituted with empty vector (delta), wild type FLAG-G9a (WT), FLAG-G9a K165A (K165A) or FLAG-G9a H1093K catalytic mutant (H1093K).
Data processing OVerall intensity of the image was targeted to 500
Probe set intensity was extracted by GCOS 1.4
 
Submission date Jun 04, 2007
Last update date Mar 18, 2009
Contact name Agnes Viale
E-mail(s) vialea@mskcc.org
Phone 212 639 3031
Fax 212 717 3053
Organization name Memorial Sloan Kettering Cancer Center
Department Molecular Biology
Lab Genomics Core Laboratory
Street address 1275 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL8321
Series (1)
GSE8011 Methylation of hMTase G9a on a histone-like site regulates protein complex assembly

Data table header descriptions
ID_REF
VALUE signal intensity
ABS_CALL absent/present call
DETECTION P-VALUE confidence on call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 147.018 P 0.000856509
AFFX-BioB-M_at 180.099 P 0.000224668
AFFX-BioB-3_at 105.539 P 0.000389797
AFFX-BioC-5_at 394.812 P 5.16732e-05
AFFX-BioC-3_at 583.253 P 4.42873e-05
AFFX-BioDn-5_at 840.589 P 5.16732e-05
AFFX-BioDn-3_at 2121.89 P 0.000169227
AFFX-CreX-5_at 4662.81 P 5.16732e-05
AFFX-CreX-3_at 5878.29 P 4.42873e-05
AFFX-DapX-5_at 16.4611 A 0.205732
AFFX-DapX-M_at 20.4238 A 0.116113
AFFX-DapX-3_at 9.41579 A 0.699394
AFFX-LysX-5_at 6.41557 A 0.368438
AFFX-LysX-M_at 17.6229 A 0.52976
AFFX-LysX-3_at 8.60414 A 0.287743
AFFX-PheX-5_at 1.18555 A 0.904333
AFFX-PheX-M_at 0.69554 A 0.960339
AFFX-PheX-3_at 9.5625 A 0.52976
AFFX-ThrX-5_at 13.2108 A 0.205732
AFFX-ThrX-M_at 4.23768 A 0.659339

Total number of rows: 22690

Table truncated, full table size 709 Kbytes.




Supplementary file Size Download File type/resource
GSM197749.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM197749.CHP.gz 6.1 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap