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Sample GSM197823 Query DataSets for GSM197823
Status Public on May 27, 2008
Title Fat line chickens, biological rep3
Sample type RNA
 
Source name fat line chicken adipose tissue at 7-week of age
Organism Gallus gallus
Characteristics Strain: Arbor Acres breed,Gender:male
Age: 49 day after birth
Treatment protocol Birds were slaughtered at 7 weeks and abdominal fat was isolated, immediately frozen in liquid nitrogen and stored at -80oC.
Growth protocol All birds were kept in similar environmental conditions and had access to feed and water ad libitum, and were fed with a commercial corn-soybean-based diet that met all NRC requirements (National Research Council, 1994). From hatching to 3 weeks the birds received a starter feed (3,100 kcal ME/kg and 210 g/kg CP), and from 4 weeks to slaughter they were fed a grower diet (3,000 kcal ME/kg and 190 g/kg CP).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description Gene expression data from chicken adipose tissue at 7-week of age
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
 
Submission date Jun 04, 2007
Last update date May 27, 2008
Contact name Hongbao Wang
Organization name Northeast Agricultural University
Street address No. 59 Mucai Street, Xiangfang District
City Harbin
ZIP/Postal code 150030
Country China
 
Platform ID GPL3213
Series (1)
GSE8010 Expression data from chicken adipose tissue at 7-week of age

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AB112960.CDS1.S1_at 41.9 A 0.27417
AF004856.CDS1.S1_s_at 18.3 A 0.533936
AF004857.CDS1.S1_s_at 20.4 A 0.633789
AF004857.CDS2.S1_s_at 27.7 A 0.80542
AF004857.CDS3.S1_s_at 55.4 A 0.246094
AF059716.CDS1.S1_at 101.7 A 0.067627
AF059717.CDS1.S1_at 7.7 A 0.919434
AF059721.CDS1.S1_at 77.6 A 0.246094
AF059724.CDS1.S1_at 19.6 A 0.80542
AF059725.CDS1.S1_at 47.3 A 0.633789
AF176590.CDS1.S1_s_at 9.3 A 0.601074
AF176591.CDS1.S1_s_at 123.4 A 0.27417
AF176592.CDS1.S1_s_at 21.3 A 0.828613
AF176592.CDS2.S1_s_at 35.7 A 0.633789
AF218359.CDS1.S1_at 35.8 A 0.303711
AF218359.CDS2.S1_at 96.5 A 0.219482
AF218359.CDS3.S1_at 53.1 A 0.095215
AF243438.CDS1.S1_s_at 107.6 A 0.334473
AF243438.CDS10.S1_s_at 3.1 A 0.828613
AF243438.CDS11.S1_s_at 42.2 A 0.780518

Total number of rows: 38535

Table truncated, full table size 1359 Kbytes.




Supplementary file Size Download File type/resource
GSM197823.CEL.gz 3.5 Mb (ftp)(http) CEL
GSM197823.EXP.gz 488 b (ftp)(http) EXP
Processed data included within Sample table

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