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Sample GSM197949 Query DataSets for GSM197949
Status Public on Mar 11, 2008
Title quail, time26, biological rep2
Sample type RNA
 
Source name Japanese quail mediobasal hypothalamus, time26
Organism Coturnix japonica
Characteristics male
Treatment protocol Animals were transferred from short day condition (6h light: 18h dark) to long day conditions (20h light: 4h dark) (1 short day and 2 long days).
Growth protocol Eight week-old male quail kept under short day condition (6h light:18h dark) for 4 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 h at 45 C on GeneChip Checken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description gene expression data of MBH at time 26
Data processing The data were analyzed with GeneChip Operating Software version 1.2 (GCOS 1.2) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 05, 2007
Last update date Mar 11, 2008
Contact name Takashi Yoshimura
E-mail(s) takashiy@agr.nagoya-u.ac.jp
Phone +81-52-789-4056
Fax +81-52-789-4056
Organization name Nagoya University
Department Graduate School of Bioagricultural Sciences
Lab Lab. of Animal Functional Genomics
Street address Furo-cho, Chikusa-ku
City Nagoya
State/province Aichi
ZIP/Postal code 464-8601
Country Japan
 
Platform ID GPL3213
Series (1)
GSE8017 Global gene expression during the photoinduction process.

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 185.562 P 0.000258358
AFFX-BioB-M_at 279.625 P 5.16732e-05
AFFX-BioB-3_at 171.578 P 4.42873e-05
AFFX-BioC-5_at 470.328 P 5.16732e-05
AFFX-BioC-3_at 535.956 P 5.16732e-05
AFFX-BioDn-5_at 1108.2 P 4.42873e-05
AFFX-BioDn-3_at 2037.65 P 5.16732e-05
AFFX-CreX-5_at 4994.86 P 5.16732e-05
AFFX-CreX-3_at 6183.51 P 4.42873e-05
AFFX-DapX-5_at 134.602 P 6.02111e-05
AFFX-DapX-M_at 295.752 P 0.000340305
AFFX-DapX-3_at 362.855 P 6.02111e-05
AFFX-LysX-5_at 18.4087 P 0.000662269
AFFX-LysX-M_at 33.9585 A 0.216524
AFFX-LysX-3_at 56.8974 P 4.42873e-05
AFFX-PheX-5_at 20.6057 P 0.000340305
AFFX-PheX-M_at 22.0737 P 0.0138105
AFFX-PheX-3_at 52.7883 P 0.00179591
AFFX-ThrX-5_at 30.3135 P 0.00110197
AFFX-ThrX-M_at 47.8404 P 0.000258358

Total number of rows: 38535

Table truncated, full table size 1472 Kbytes.




Supplementary file Size Download File type/resource
GSM197949.CEL.gz 5.1 Mb (ftp)(http) CEL
GSM197949.CHP.gz 4.4 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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