|
| Status |
Public on Mar 11, 2008 |
| Title |
quail, time30, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Japanese quail mediobasal hypothalamus, time30
|
| Organism |
Coturnix japonica |
| Characteristics |
male
|
| Treatment protocol |
Animals were transferred from short day condition (6h light: 18h dark) to long day conditions (20h light: 4h dark) (1 short day and 2 long days).
|
| Growth protocol |
Eight week-old male quail kept under short day condition (6h light:18h dark) for 4 weeks were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 h at 45 C on GeneChip Checken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
gene expression data of MBH at time 30
|
| Data processing |
The data were analyzed with GeneChip Operating Software version 1.2 (GCOS 1.2) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jun 05, 2007 |
| Last update date |
Mar 11, 2008 |
| Contact name |
Takashi Yoshimura |
| E-mail(s) |
takashiy@agr.nagoya-u.ac.jp
|
| Phone |
+81-52-789-4056
|
| Fax |
+81-52-789-4056
|
| Organization name |
Nagoya University
|
| Department |
Graduate School of Bioagricultural Sciences
|
| Lab |
Lab. of Animal Functional Genomics
|
| Street address |
Furo-cho, Chikusa-ku
|
| City |
Nagoya |
| State/province |
Aichi |
| ZIP/Postal code |
464-8601 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE8017 |
Global gene expression during the photoinduction process. |
|
