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Sample GSM197960 Query DataSets for GSM197960
Status Public on Mar 11, 2008
Title quail, time42, biological rep1
Sample type RNA
 
Source name Japanese quail mediobasal hypothalamus, time42
Organism Coturnix japonica
Characteristics male
Treatment protocol Animals were transferred from short day condition (6h light: 18h dark) to long day conditions (20h light: 4h dark) (1 short day and 2 long days).
Growth protocol Eight week-old male quail kept under short day condition (6h light:18h dark) for 4 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 h at 45 C on GeneChip Checken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description gene expression data of MBH at time 42
Data processing The data were analyzed with GeneChip Operating Software version 1.2 (GCOS 1.2) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 05, 2007
Last update date Mar 11, 2008
Contact name Takashi Yoshimura
E-mail(s) takashiy@agr.nagoya-u.ac.jp
Phone +81-52-789-4056
Fax +81-52-789-4056
Organization name Nagoya University
Department Graduate School of Bioagricultural Sciences
Lab Lab. of Animal Functional Genomics
Street address Furo-cho, Chikusa-ku
City Nagoya
State/province Aichi
ZIP/Postal code 464-8601
Country Japan
 
Platform ID GPL3213
Series (1)
GSE8017 Global gene expression during the photoinduction process.

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 168.662 P 0.000296708
AFFX-BioB-M_at 246.761 P 4.42873e-05
AFFX-BioB-3_at 170.344 P 4.42873e-05
AFFX-BioC-5_at 424.967 P 5.16732e-05
AFFX-BioC-3_at 463.394 P 4.42873e-05
AFFX-BioDn-5_at 967.155 P 4.42873e-05
AFFX-BioDn-3_at 1780.93 P 5.16732e-05
AFFX-CreX-5_at 4474.86 P 4.42873e-05
AFFX-CreX-3_at 5427.2 P 4.42873e-05
AFFX-DapX-5_at 94.9129 P 7.00668e-05
AFFX-DapX-M_at 244.313 P 0.000581214
AFFX-DapX-3_at 336.827 P 5.16732e-05
AFFX-LysX-5_at 15.9948 P 0.000258067
AFFX-LysX-M_at 20.8867 A 0.340661
AFFX-LysX-3_at 61.2142 P 0.00010954
AFFX-PheX-5_at 19.9251 P 0.000340305
AFFX-PheX-M_at 17.5229 P 0.0336296
AFFX-PheX-3_at 40.0163 P 0.000972149
AFFX-ThrX-5_at 27.2108 P 0.00556451
AFFX-ThrX-M_at 41.3617 P 0.000753643

Total number of rows: 38535

Table truncated, full table size 1471 Kbytes.




Supplementary file Size Download File type/resource
GSM197960.CEL.gz 5.1 Mb (ftp)(http) CEL
GSM197960.CHP.gz 4.4 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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