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Sample GSM197962 Query DataSets for GSM197962
Status Public on Mar 11, 2008
Title quail, time44, biological rep1
Sample type RNA
 
Source name Japanese quail mediobasal hypothalamus, time44
Organism Coturnix japonica
Characteristics male
Treatment protocol Animals were transferred from short day condition (6h light: 18h dark) to long day conditions (20h light: 4h dark) (1 short day and 2 long days).
Growth protocol Eight week-old male quail kept under short day condition (6h light:18h dark) for 4 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 h at 45 C on GeneChip Checken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description gene expression data of MBH at time 44
Data processing The data were analyzed with GeneChip Operating Software version 1.2 (GCOS 1.2) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 05, 2007
Last update date Mar 11, 2008
Contact name Takashi Yoshimura
E-mail(s) takashiy@agr.nagoya-u.ac.jp
Phone +81-52-789-4056
Fax +81-52-789-4056
Organization name Nagoya University
Department Graduate School of Bioagricultural Sciences
Lab Lab. of Animal Functional Genomics
Street address Furo-cho, Chikusa-ku
City Nagoya
State/province Aichi
ZIP/Postal code 464-8601
Country Japan
 
Platform ID GPL3213
Series (1)
GSE8017 Global gene expression during the photoinduction process.

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 184.355 P 0.000340305
AFFX-BioB-M_at 326.368 P 4.42873e-05
AFFX-BioB-3_at 237.929 P 4.42873e-05
AFFX-BioC-5_at 515.097 P 5.16732e-05
AFFX-BioC-3_at 590.658 P 4.42873e-05
AFFX-BioDn-5_at 1246.31 P 4.42873e-05
AFFX-BioDn-3_at 2175.83 P 5.16732e-05
AFFX-CreX-5_at 6786.96 P 5.16732e-05
AFFX-CreX-3_at 7191.09 P 4.42873e-05
AFFX-DapX-5_at 108.871 P 7.00668e-05
AFFX-DapX-M_at 265.332 P 0.000581214
AFFX-DapX-3_at 358.257 P 5.16732e-05
AFFX-LysX-5_at 18.6721 P 0.00159257
AFFX-LysX-M_at 31.0447 A 0.205732
AFFX-LysX-3_at 64.8451 P 0.000195116
AFFX-PheX-5_at 18.8369 P 0.000581214
AFFX-PheX-M_at 29.758 P 0.00844047
AFFX-PheX-3_at 44.377 P 0.000972149
AFFX-ThrX-5_at 28.4095 P 0.002867
AFFX-ThrX-M_at 43.6212 P 0.000224668

Total number of rows: 38535

Table truncated, full table size 1473 Kbytes.




Supplementary file Size Download File type/resource
GSM197962.CEL.gz 5.1 Mb (ftp)(http) CEL
GSM197962.CHP.gz 4.4 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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