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Sample GSM197999 Query DataSets for GSM197999
Status Public on Mar 11, 2008
Title quail, long day condition at ZT14, biological rep1
Sample type RNA
 
Source name mediobasal hypothalamus, long day condition (20h light:4h dark), 2week
Organism Coturnix japonica
Characteristics male
Treatment protocol At the age of 8 weeks old, birds were devided into two groups. One group was continuously kept under short day condition (6h light:18h dark) for 2 weeks. And the other group was transferred to long day condition (20h light: 4h dark) for 2 weeks.
Growth protocol Eight week-old male quail kept under short day condition (6h light:18h dark) for 4 weeks were used.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Trizol according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 h at 45 C on GeneChip Checken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
Description gene expression data of MBH at long day condition ZT14
Data processing The data were analyzed with GeneChip Operating Software version 1.2 (GCOS 1.2) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jun 05, 2007
Last update date Mar 11, 2008
Contact name Takashi Yoshimura
E-mail(s) takashiy@agr.nagoya-u.ac.jp
Phone +81-52-789-4056
Fax +81-52-789-4056
Organization name Nagoya University
Department Graduate School of Bioagricultural Sciences
Lab Lab. of Animal Functional Genomics
Street address Furo-cho, Chikusa-ku
City Nagoya
State/province Aichi
ZIP/Postal code 464-8601
Country Japan
 
Platform ID GPL3213
Series (1)
GSE8018 Gene expression in the quail MBH under short and long day conditions.

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 174.434 P 0.000753643
AFFX-BioB-M_at 227.26 P 6.02111e-05
AFFX-BioB-3_at 153.315 P 6.02111e-05
AFFX-BioC-5_at 487.811 P 5.16732e-05
AFFX-BioC-3_at 624.702 P 4.42873e-05
AFFX-BioDn-5_at 1282.89 P 4.42873e-05
AFFX-BioDn-3_at 2087.34 P 6.02111e-05
AFFX-CreX-5_at 5523.93 P 5.16732e-05
AFFX-CreX-3_at 6447.09 P 4.42873e-05
AFFX-DapX-5_at 119.642 P 0.000126798
AFFX-DapX-M_at 349.887 P 0.000509415
AFFX-DapX-3_at 510.257 P 5.16732e-05
AFFX-LysX-5_at 18.2667 P 0.00159257
AFFX-LysX-M_at 36.0719 A 0.147939
AFFX-LysX-3_at 77.8491 P 9.4506e-05
AFFX-PheX-5_at 31.175 P 0.00762003
AFFX-PheX-M_at 49.8644 P 0.000509415
AFFX-PheX-3_at 63.1279 P 0.000581214
AFFX-ThrX-5_at 34.5998 P 0.0113844
AFFX-ThrX-M_at 64.1924 P 4.42873e-05

Total number of rows: 38535

Table truncated, full table size 1474 Kbytes.




Supplementary file Size Download File type/resource
GSM197999.CEL.gz 4.3 Mb (ftp)(http) CEL
GSM197999.CHP.gz 4.4 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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