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Sample GSM1981362 Query DataSets for GSM1981362
Status Public on Dec 25, 2015
Title HSCs, ICG-001 treatment
Sample type RNA
 
Source name HSCs, ICG-001 treatment
Organism Rattus norvegicus albus
Characteristics cell type: primary hepatic stellate cells (HSCs)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from HSCs using the RNeasy Mini Kit (Qiagen) according to the manufacturer's instructions.
Label biotin
Label protocol Ambion WT Expression Kit was used to generate ssDNA targets. Fragmentation and biotin labeling were performed using the Affymetrix WT Terminal Labeling and Hybridization Kit.
 
Hybridization protocol The fragmented and labeled targets were hybridized to the arrays per standard Affymetrix protocol in an Affymetrix GeneChip Hybridization Oven 640. The arrays were then washed and stained in an Affymetrix GeneChip Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data
Data processing Data were analyzed with Affymetrix Expression Console using Robust Multichip Average (RMA) using Affymetrix default analysis settings.
 
Submission date Dec 24, 2015
Last update date Dec 25, 2015
Contact name Hidekazu Tsukamoto
E-mail(s) htsukamo@med.usc.edu
Organization name University of Southern California
Department Pathology
Lab Southern California Research Center for ALPD & Cirrhosis
Street address 1333 San Pablo Street MMR414
City Los Angeles
State/province CA
ZIP/Postal code 90089
Country USA
 
Platform ID GPL6247
Series (1)
GSE76330 Global analysis of genes regulated by canonical Wnt pathway

Data table header descriptions
ID_REF
VALUE RMA quantile gene expression data.

Data table
ID_REF VALUE
10701620 5.66650
10701630 4.22567
10701632 5.40699
10701636 4.65506
10701643 4.74078
10701648 4.87050
10701654 5.68176
10701663 7.04849
10701666 6.22717
10701668 5.46797
10701671 4.72736
10701674 5.63473
10701679 7.80496
10701684 5.95081
10701689 8.94276
10701691 6.03528
10701697 6.59819
10701699 6.96555
10701709 10.50941
10701714 7.25950

Total number of rows: 26988

Table truncated, full table size 451 Kbytes.




Supplementary file Size Download File type/resource
GSM1981362_O588-A-RaGene-1_0-st-v1-01-1_ICG-3_.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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