Total RNA was isolated from each tissue using the Rneasy Total RNA Isolation kit (Qiagen, Valencia, CA).
Label
biotin
Label protocol
5 μg total RNA was labeled with biotin according to standard protocols (affymetrix, Santa Clara, CA).
Hybridization protocol
10 μg biotinylated cRNA was hybridized to mouse Affymetrix U430 2.0 chips. Hybridized chips were stained and washed on an Affymetrix FS400 fluidics station using the Affymetrix antibody amplification protocol.
Scan protocol
Chips were scanned using an Affymetrix Gene Array 2500 Scanner.
Description
non-transgenic mouse
Data processing
The Rosetta Resolver System 5.1 (Rosetta Biosoftware, Seattle, Washington) was used for data normalization and analysis.
