|
| Status |
Public on Oct 30, 2007 |
| Title |
T4 CD2-CD30L peripheral lymph node |
| Sample type |
RNA |
| |
|
| Source name |
Peripheral lymph node, CD30L transgenic mouse # 4
|
| Organism |
Mus musculus |
| Characteristics |
Gender: Female, Age: 12 weeks, Strain: C57BL/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from each tissue using the Rneasy Total RNA Isolation kit (Qiagen, Valencia, CA).
|
| Label |
biotin
|
| Label protocol |
5 μg total RNA was labeled with biotin according to standard protocols (affymetrix, Santa Clara, CA).
|
| |
|
| Hybridization protocol |
10 μg biotinylated cRNA was hybridized to mouse Affymetrix U430 2.0 chips. Hybridized chips were stained and washed on an Affymetrix FS400 fluidics station using the Affymetrix antibody amplification protocol.
|
| Scan protocol |
Chips were scanned using an Affymetrix Gene Array 2500 Scanner.
|
| Description |
Transgenic mice were generated with constitutive T cellspecific overexpression of CD30L as a tool to help unravel the consequences of CD30/CD30L interactions in vivo.
|
| Data processing |
The Rosetta Resolver System 5.1 (Rosetta Biosoftware, Seattle, Washington) was used for data normalization and analysis.
|
| |
|
| Submission date |
Jun 06, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
John Gosink |
| E-mail(s) |
gosinkj@amgen.com
|
| Phone |
206 265-8217
|
| Organization name |
Amgen
|
| Street address |
1201 Amgen Court West
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98119 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8039 |
Gene Expression in Transgenic Mice Expressing CD30L on T Cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
