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Sample GSM201830 Query DataSets for GSM201830
Status Public on Nov 10, 2008
Title Muscle PCOS pioglitazon 251
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and constant normalization was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test for paired data was used to calculate p-values
 
Submission date Jun 16, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE8157 Gene expression profiling in skeletal muscle of PCOS after pioglitazone therapy
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Normalized expression value

Data table
ID_REF VALUE
1570117_at 131.7433288
1552518_s_at 73.98574415
204714_s_at 213.7677009
204991_s_at 146.8309788
1567056_at 110.8030013
1553686_at 191.4058152
208554_at 99.88543515
1561854_at 93.95929363
214402_s_at 195.2370618
204734_at 135.5723546
1560047_s_at 538.782596
1555558_at 91.21072829
206627_s_at 90.18336311
238720_at 87.42048081
217675_at 94.61419046
1557094_at 89.95864574
207363_at 227.913881
237566_at 109.1592944
230197_s_at 230.5862845
242562_at 69.65288392

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM201830.CEL.gz 8.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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