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Sample GSM201831 Query DataSets for GSM201831
Status Public on Nov 10, 2008
Title Muscle PCOS pioglitazone 271
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and constant normalization was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test for paired data was used to calculate p-values
 
Submission date Jun 16, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE8157 Gene expression profiling in skeletal muscle of PCOS after pioglitazone therapy
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Normalized expression value

Data table
ID_REF VALUE
1570117_at 104.1484912
1552518_s_at 77.93646958
204714_s_at 279.0512381
204991_s_at 112.2500684
1567056_at 81.86432728
1553686_at 141.8692287
208554_at 64.00249901
1561854_at 48.71341994
214402_s_at 136.2895075
204734_at 119.692626
1560047_s_at 571.7576127
1555558_at 62.56803407
206627_s_at 78.179527
238720_at 94.36727816
217675_at 67.73106907
1557094_at 76.87661662
207363_at 221.5236721
237566_at 92.48638143
230197_s_at 199.2921774
242562_at 58.35594925

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM201831.CEL.gz 8.3 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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