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Sample GSM201834 Query DataSets for GSM201834
Status Public on Nov 10, 2008
Title Muscle PCOS pioglitazone 81
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and constant normalization was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test for paired data was used to calculate p-values
 
Submission date Jun 16, 2007
Last update date Aug 14, 2011
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE8157 Gene expression profiling in skeletal muscle of PCOS after pioglitazone therapy

Data table header descriptions
ID_REF
VALUE Normalized expression value

Data table
ID_REF VALUE
1570117_at 86.09295771
1552518_s_at 48.08300976
204714_s_at 289.2254329
204991_s_at 118.1550644
1567056_at 64.79669823
1553686_at 137.3585997
208554_at 58.69879527
1561854_at 23.57653188
214402_s_at 99.86310285
204734_at 92.04632887
1560047_s_at 706.8839317
1555558_at 44.27943637
206627_s_at 43.86934712
238720_at 68.9036444
217675_at 59.62183442
1557094_at 47.82844752
207363_at 154.827026
237566_at 102.4675385
230197_s_at 154.7660235
242562_at 42.0572105

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM201834.CEL.gz 8.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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