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Sample GSM201843 Query DataSets for GSM201843
Status Public on Nov 10, 2008
Title Muscle PCOS after pioglitazone 42
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and constant normalization was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test for paired data was used to calculate p-values
 
Submission date Jun 16, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE8157 Gene expression profiling in skeletal muscle of PCOS after pioglitazone therapy
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Normalized expression value

Data table
ID_REF VALUE
1570117_at 20.49305365
1552518_s_at 12.61299678
204714_s_at 57.88172799
204991_s_at 43.35567955
1567056_at 21.70106143
1553686_at 46.32600961
208554_at 22.2950433
1561854_at 11.96420387
214402_s_at 37.139666
204734_at 26.91330402
1560047_s_at 234.7606823
1555558_at 17.72575024
206627_s_at 16.47335406
238720_at 28.37857479
217675_at 15.65891636
1557094_at 16.38282303
207363_at 43.76133319
237566_at 29.97195199
230197_s_at 53.49427834
242562_at 12.42922605

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM201843.CEL.gz 8.0 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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