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Sample GSM201854 Query DataSets for GSM201854
Status Public on Nov 10, 2008
Title Muscle PCOS control 9
Sample type RNA
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and constant normalization was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test for paired data was used to calculate p-values
Submission date Jun 17, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
Platform ID GPL570
Series (1)
GSE8157 Gene expression profiling in skeletal muscle of PCOS after pioglitazone therapy
Reanalyzed by GSE119087

Data table header descriptions
VALUE Normalized expression value

Data table
1007_s_at 566.2039315
1053_at 79.02797253
117_at 185.6122728
121_at 722.0576346
1255_g_at 35.0221855
1294_at 154.5705566
1316_at 479.488051
1320_at 99.03569388
1405_i_at 68.99989405
1431_at 231.6516387
1438_at 133.7980391
1487_at 699.5868047
1494_f_at 267.3271823
1552256_a_at 164.7780838
1552257_a_at 141.2171375
1552258_at 80.50096917
1552261_at 284.7070774
1552263_at 44.5052938
1552264_a_at 60.27981303
1552266_at 28.59330494

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.

Supplementary file Size Download File type/resource
GSM201854.CEL.gz 8.1 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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