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Sample GSM201869 Query DataSets for GSM201869
Status Public on Nov 10, 2008
Title Muscle PCOS case 201
Sample type RNA
 
Source name Vastus lateralis muscle
Organism Homo sapiens
Characteristics Vastus lateralis muscle
Extracted molecule total RNA
Extraction protocol Frozen muscle tissue was homogenized in Trizol with a Polytron Homogenizer and total RNA was purified using TRIzol Reagent including an extra step after phase separation with chloroform. Briefly, 1 volume of phenol-chloroform-isoamylalcohol (25-24-1) was added to the aqueous phase and spun at 10.000 x g for 10 minutes at 4˚C. Then, 1 volume of chloroform was added to the aqueous phase and spun at 12.000 x g for 20 minutes at 4˚C before RNA precipitation.
Label Biotin
Label protocol One µg purified total RNA was converted to biotin-labeled aRNA using the MessageAmpTM II-Biotin single round aRNA amplification kit.
 
Hybridization protocol Standard Affymetrix protocol
Scan protocol Standard Affymetrix protocol
Description Vastus lateralis muscle
Data processing The R statistical software was applied for data analysis. The RMA method was used for global background correction and constant normalization was used for normalization. Gene expression index calculation was done using model based index calculation (MBEI). Only perfect match probes were included in data analysis. Welch two sample t-test for paired data was used to calculate p-values
 
Submission date Jun 18, 2007
Last update date Aug 28, 2018
Contact name Vibe Skov
E-mail(s) vihs@regionsjaelland.dk
Phone +4526178735
Organization name Roskilde Hospital
Department Hematology
Street address Koegevej 7-13
City Roskilde
ZIP/Postal code 4000
Country Denmark
 
Platform ID GPL570
Series (1)
GSE8157 Gene expression profiling in skeletal muscle of PCOS after pioglitazone therapy
Relations
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE Normalized expression value

Data table
ID_REF VALUE
1007_s_at 615.3929321
1053_at 107.5515647
117_at 173.964538
121_at 1012.99552
1255_g_at 42.3393194
1294_at 168.2975828
1316_at 614.5096653
1320_at 128.8124761
1405_i_at 113.7663287
1431_at 233.1127441
1438_at 174.1840229
1487_at 693.3763263
1494_f_at 342.3390337
1552256_a_at 246.342501
1552257_a_at 151.0485343
1552258_at 71.51411064
1552261_at 535.6739191
1552263_at 35.63261096
1552264_a_at 69.25189719
1552266_at 26.00597715

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.




Supplementary file Size Download File type/resource
GSM201869.CEL.gz 8.3 Mb (ftp)(http) CEL
Processed data included within Sample table
Raw data provided as supplementary file

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