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Sample GSM202328 Query DataSets for GSM202328
Status Public on Dec 31, 2009
Title Sustained L-type Calcium Channel Blockade Alters Gene Regulation in the Adult Mouse Ventricle-VER1
Sample type RNA
Source name heart, left ventricle, free wall, 24 hours verapmil (3.6 mg/Kg/day) treatment
Organism Mus musculus
Characteristics Strain: ICR
Gender: Female
Age: 12-14 weeks
Tissue: Heart/ left ventricle free wall
Treatment: 24 hours verapamil (3.6 mg/kg)
Biomaterial provider Elizabeth Schroder
Treatment protocol Female ICR mice (12-14 weeks age) weighing between 25 and 30 grams were anesthetized with a ketamine/xylazine mixture ( i.p.) allowing the subcutaneous implantation of miniosmotic pumps (Alzet, model 2001). The pumps were filled with verapamil (3.6 mg/kg/day). After treatment animals were anesthetized and weighed. Hearts were excised, rinsed, blotted dry, weighed, and then frozen on dry ice and the stored at -80oC until studied. Animals were anesthetized and euthanized according to animal protocols approved by the University of Kentucky Institutional Animal Care and Use Committee. This investigation conforms with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication NO. 85-23, revised 1996).
Extracted molecule total RNA
Extraction protocol Left ventricular free wall from female mice was rapidly excised and either snap frozen at -80oC or used immediately for RNA isolation. Total RNA was isolated using the RNAqueous -4PCR kit (Ambion) and quantitated spectrophotometrically at 260nm. Contaminating genomic DNA was eliminated by DNase treatment (Ambion). RNA quality was assessed using the Agilent 2100 Bioanalyzer.
Label biotin
Label protocol standard Affymetrix procedures

Hybridization protocol standard Affymetrix procedures

Scan protocol standard Affymetrix procedures

Description verapamil treated heart tissue
Data processing GeneChip Operating Software
Submission date Jun 19, 2007
Last update date Aug 14, 2011
Contact name Elizabeth Anne Schroder
Phone 859-323-2918
Organization name University of Kentucky
Department Physiology
Lab Satin Lab
Street address 800 Rose Street, MS508
City Lexington
State/province KY
ZIP/Postal code 40536-0298
Country USA
Platform ID GPL1261
Series (1)
GSE8367 Sustained L-type Calcium Channel Blockade Alters Gene Regulation in the Adult Mouse Ventricle

Data table header descriptions
VALUE Calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 507.799 P 0.000340305
AFFX-BioB-M_at 1063.07 P 4.42873e-05
AFFX-BioB-3_at 625.671 P 0.000195116
AFFX-BioC-5_at 1799.65 P 5.16732e-05
AFFX-BioC-3_at 1475.21 P 4.42873e-05
AFFX-BioDn-5_at 2922.43 P 4.42873e-05
AFFX-BioDn-3_at 9629.84 P 5.16732e-05
AFFX-CreX-5_at 21373.9 P 5.16732e-05
AFFX-CreX-3_at 22718.2 P 4.42873e-05
AFFX-DapX-5_at 7.50998 A 0.425962
AFFX-DapX-M_at 12.0722 A 0.699394
AFFX-DapX-3_at 17.0961 A 0.749204
AFFX-LysX-5_at 18.8629 A 0.382599
AFFX-LysX-M_at 12.2609 A 0.686277
AFFX-LysX-3_at 34.6769 A 0.0894781
AFFX-PheX-5_at 1.71954 A 0.99156
AFFX-PheX-M_at 3.59982 A 0.910522
AFFX-PheX-3_at 27.9699 A 0.368438
AFFX-ThrX-5_at 7.86026 A 0.843268
AFFX-ThrX-M_at 27.4001 A 0.275146

Total number of rows: 45101

Table truncated, full table size 1378 Kbytes.

Supplementary file Size Download File type/resource
GSM202328.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM202328.CHP.gz 243.9 Kb (ftp)(http) CHP
Raw data included within Sample table
Processed data included within Sample table
Raw data provided as supplementary file
Processed data provided as supplementary file

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