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Sample GSM202341 Query DataSets for GSM202341
Status Public on Dec 31, 2009
Title Sustained L-type Calcium Channel Blockade Alters Gene Regulation in the Adult Mouse Ventricle-male vehicle1
Sample type RNA
 
Source name heart, left ventricle, free wall, 24 hours vehicle (0.02% ascorbic acid) treatment
Organism Mus musculus
Characteristics Strain: ICR
Gender: Male
Age: 12-14 weeks
Tissue: Heart/ left ventricle free wall
Treatment: 24 hours vehicle (0.02% ascorbic acid)
Biomaterial provider Elizabeth Schroder
Treatment protocol Male ICR mice (12-14 weeks age) weighing between 25 and 30 grams were anesthetized with a ketamine/xylazine mixture ( i.p.) allowing the subcutaneous implantation of miniosmotic pumps (Alzet, model 2001). The pumps were filled with vehicle (0.02% ascorbic acid). After treatment animals were anesthetized and weighed. Hearts were excised, rinsed, blotted dry, weighed, and then frozen on dry ice and the stored at -80oC until studied. Animals were anesthetized and euthanized according to animal protocols approved by the University of Kentucky Institutional Animal Care and Use Committee. This investigation conforms with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication NO. 85-23, revised 1996).
Extracted molecule total RNA
Extraction protocol Left ventricular free wall from male mice was rapidly excised and either snap frozen at -80oC or used immediately for RNA isolation. Total RNA was isolated using the RNAqueous -4PCR kit (Ambion) and quantitated spectrophotometrically at 260nm. Contaminating genomic DNA was eliminated by DNase treatment (Ambion). RNA quality was assessed using the Agilent 2100 Bioanalyzer.
Label biotin
Label protocol standard Affymetrix procedures
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description vehicle treated heart tissue
Data processing GeneChip Operating Software
 
Submission date Jun 19, 2007
Last update date Aug 14, 2011
Contact name Elizabeth Anne Schroder
E-mail(s) eschr0@uky.edu
Phone 859-323-2918
Organization name University of Kentucky
Department Physiology
Lab Satin Lab
Street address 800 Rose Street, MS508
City Lexington
State/province KY
ZIP/Postal code 40536-0298
Country USA
 
Platform ID GPL1261
Series (1)
GSE8367 Sustained L-type Calcium Channel Blockade Alters Gene Regulation in the Adult Mouse Ventricle

Data table header descriptions
ID_REF
VALUE calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 726.975 P 0.000509415
AFFX-BioB-M_at 1527.67 P 4.42873e-05
AFFX-BioB-3_at 866.997 P 6.02111e-05
AFFX-BioC-5_at 2091.67 P 6.02111e-05
AFFX-BioC-3_at 2385.44 P 4.42873e-05
AFFX-BioDn-5_at 4171.48 P 4.42873e-05
AFFX-BioDn-3_at 10979.9 P 4.42873e-05
AFFX-CreX-5_at 25723 P 4.42873e-05
AFFX-CreX-3_at 26980.1 P 4.42873e-05
AFFX-DapX-5_at 9.8684 A 0.686277
AFFX-DapX-M_at 19.0571 A 0.686277
AFFX-DapX-3_at 38.5323 A 0.287743
AFFX-LysX-5_at 21.6981 A 0.300606
AFFX-LysX-M_at 36.1572 A 0.455413
AFFX-LysX-3_at 21.4225 A 0.175328
AFFX-PheX-5_at 5.4893 A 0.58862
AFFX-PheX-M_at 2.07238 A 0.98333
AFFX-PheX-3_at 15.2512 A 0.41138
AFFX-ThrX-5_at 4.06394 A 0.963431
AFFX-ThrX-M_at 14.9868 A 0.52976

Total number of rows: 45101

Table truncated, full table size 1378 Kbytes.




Supplementary file Size Download File type/resource
GSM202341.CEL.gz 3.7 Mb (ftp)(http) CEL
GSM202341.CHP.gz 244.5 Kb (ftp)(http) CHP
Raw data included within Sample table
Processed data included within Sample table
Raw data provided as supplementary file
Processed data provided as supplementary file

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