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Sample GSM202345 Query DataSets for GSM202345
Status Public on Dec 31, 2009
Title Sustained L-type Calcium Channel Blockade Alters Gene Regulation in the Adult Mouse Ventricle- male verapamil 1
Sample type RNA
Source name heart, left ventricle, free wall, 24 hours verapamil (3.6 mg/kg/day) treatment
Organism Mus musculus
Characteristics Strain: ICR
Gender: Male
Age: 12-14 weeks
Tissue: Heart/ left ventricle free wall
Treatment: 24 hours verapamil (3.6 mg/kg/day)
Biomaterial provider Elizabeth Schroder
Treatment protocol Male ICR mice (12-14 weeks age) weighing between 25 and 30 grams were anesthetized with a ketamine/xylazine mixture ( i.p.) allowing the subcutaneous implantation of miniosmotic pumps (Alzet, model 2001). The pumps were filled with verapamil (3.6 mg/kg/day). After treatment animals were anesthetized and weighed. Hearts were excised, rinsed, blotted dry, weighed, and then frozen on dry ice and the stored at -80oC until studied. Animals were anesthetized and euthanized according to animal protocols approved by the University of Kentucky Institutional Animal Care and Use Committee. This investigation conforms with the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication NO. 85-23, revised 1996).
Extracted molecule total RNA
Extraction protocol Left ventricular free wall from male mice was rapidly excised and either snap frozen at -80oC or used immediately for RNA isolation. Total RNA was isolated using the RNAqueous -4PCR kit (Ambion) and quantitated spectrophotometrically at 260nm. Contaminating genomic DNA was eliminated by DNase treatment (Ambion). RNA quality was assessed using the Agilent 2100 Bioanalyzer.
Label biotin
Label protocol standard Affymetrix procedures
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description verapamil treated heart tissue
Data processing GeneChip Operating Software
Submission date Jun 19, 2007
Last update date Aug 14, 2011
Contact name Elizabeth Anne Schroder
Phone 859-323-2918
Organization name University of Kentucky
Department Physiology
Lab Satin Lab
Street address 800 Rose Street, MS508
City Lexington
State/province KY
ZIP/Postal code 40536-0298
Country USA
Platform ID GPL1261
Series (1)
GSE8367 Sustained L-type Calcium Channel Blockade Alters Gene Regulation in the Adult Mouse Ventricle

Data table header descriptions
VALUE calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 603.087 P 0.000662269
AFFX-BioB-M_at 900.096 P 4.42873e-05
AFFX-BioB-3_at 568.556 P 8.14279e-05
AFFX-BioC-5_at 1781.48 P 7.00668e-05
AFFX-BioC-3_at 1887.66 P 4.42873e-05
AFFX-BioDn-5_at 3487.07 P 4.42873e-05
AFFX-BioDn-3_at 9402.01 P 5.16732e-05
AFFX-CreX-5_at 19992.5 P 5.16732e-05
AFFX-CreX-3_at 23296.5 P 4.42873e-05
AFFX-DapX-5_at 19.8022 A 0.313723
AFFX-DapX-M_at 32.5311 A 0.440646
AFFX-DapX-3_at 20.0344 A 0.39692
AFFX-LysX-5_at 12.1134 A 0.5
AFFX-LysX-M_at 9.16832 A 0.794268
AFFX-LysX-3_at 6.32804 A 0.440646
AFFX-PheX-5_at 2.04129 A 0.921998
AFFX-PheX-M_at 4.17149 A 0.834139
AFFX-PheX-3_at 16.2791 A 0.544587
AFFX-ThrX-5_at 4.86659 A 0.984817
AFFX-ThrX-M_at 4.91321 A 0.783476

Total number of rows: 45101

Table truncated, full table size 1378 Kbytes.

Supplementary file Size Download File type/resource
GSM202345.CEL.gz 3.8 Mb (ftp)(http) CEL
GSM202345.CHP.gz 243.9 Kb (ftp)(http) CHP
Raw data included within Sample table
Processed data included within Sample table
Raw data provided as supplementary file
Processed data provided as supplementary file

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