|
| Status |
Public on Jan 21, 2016 |
| Title |
Bdh2 morphant expressing ectopic Bdh2, rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
Bdh2 morphant zebrafish embryos expressing ectopic Bdh2 at 72 hpf
|
| Organism |
Danio rerio |
| Characteristics |
developmental stage: embryo
|
| Treatment protocol |
Fertilized 1- to 2-cell embryos were microinjected with morpholinos (MOs; 1 to 2 nL of 130 micromol/L in water; Gene Tools). Rescue experiments were performed by co-injecting 1 to 2 nL of a synthetic capped mRNA (50 microgram/ml in water) synthesized from a full-length mouse bdh2 cDNA cloned into pCS2+MT vector as described in Cell 141:1006-1017.
|
| Growth protocol |
Wild-type Danio rerio (zebrafish) were bred and raised following the procedures approved by the Case Western Reserve University Institutional Animal Care and Use Committee. The embryos were collected from natural spawnings, cultured, and staged as described in Cell 141:1006-1017.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNAs were performed using RNeasy Mini kit (Qiagen) per manufacturer's protocol.
|
| Label |
biotin
|
| Label protocol |
Per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility.
|
| |
|
| Hybridization protocol |
Per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility.
|
| Scan protocol |
Per manufacturer’s instructions (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetric, Inc., Santa Clara, CA, USA) at Case Comprehensive Cancer Center Microarray Core Facility.
|
| Data processing |
The data were analyzed with Affymetrix Expression Console Software using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 04, 2016 |
| Last update date |
Jan 21, 2016 |
| Contact name |
Zhuoming Liu |
| Organization name |
Case Western Reserve University
|
| Street address |
2103 Cornell Road
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE76509 |
Comparison of expression data between control and Bdh2 morphant zebrafish embryos, as well as Bdh2 morphant zebrafish embryos expressing ectopic Bdh2 |
|
