|
| Status |
Public on Feb 24, 2016 |
| Title |
RAW 264.7, Control 3 |
| Sample type |
RNA |
| |
|
| Source name |
RAW264.7 Macrophages
|
| Organism |
Mus musculus |
| Characteristics |
cell type: RAW264.7 Macrophages
|
| Treatment protocol |
For the experiments, lipopolysaccharide (LPS, Sigma) was used at a final concentration of 10ng/mL.
|
| Growth protocol |
The murine RAW264.7 macrophage cell line was cultured in DMEM medium (Invitrogen, Life Technologies, Carlsbad, California) with 10% Fetal Bovine Serum (FBS), 0.1mg/mL streptomycin (Invitrogen, Life Technologies, Carlsbad, California) and 100U/mL penicillin (Invitrogen, Life Technologies, Carlsbad, California).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was purified from cultured cells using the Qiagen RNeasy Mini Kit (Qiagen) according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
The Ambion® WT Expression Kit For Affymetrix® GeneChip® Whole Transcript (WT) Expression Arrays Part Number 4425209 Rev. C 09/2009
|
| |
|
| Hybridization protocol |
GeneChip® WT Terminal Labeling and Hybridization User Manual for use with the Ambion® WT Expression Kit P/N 702808 Rev. 6
|
| Scan protocol |
Affymetrix® GeneChip® Command Console® User Manual (P/N 702569).
|
| Data processing |
The data were preprocessed, normalised and analysed using the GCRMA and limma packages from R/Bioconductor software environment. The CDF was used according to GPL18802 and submitted the unique list of IDs and their expression values.
|
| |
|
| Submission date |
Jan 05, 2016 |
| Last update date |
Feb 24, 2016 |
| Contact name |
Aravind Tallam |
| E-mail(s) |
aravind.tallam@twincore.de
|
| Phone |
004917670033553
|
| Organization name |
TWINCORE
|
| Department |
BIOM
|
| Street address |
Feodor-Lynen str. 7
|
| City |
Hannover |
| State/province |
Lower Saxony |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL18802 |
| Series (2) |
| GSE76562 |
LPS stimulation of Mouse (RAW 264.7) macrophages |
| GSE76563 |
The study of inflammatory responses in mammalian macrophages with LPS stimulation |
|
