|
| Status |
Public on Aug 02, 2007 |
| Title |
Ovary_PD7_Foxo3 Wt_Replica 1 |
| Sample type |
RNA |
| |
|
| Source name |
Ovary sample from PD7 Foxo3 wild-type
|
| Organism |
Mus musculus |
| Characteristics |
PD7 Foxo3 +/+ ovary
|
| Biomaterial provider |
Castrillon Laboratory
|
| Treatment protocol |
Female pups were sacrificed by decapitation and ovaries were immediately removed to RNase-free PBS. Bursa and oviduct material was removed and the ovaries were transferred to Tripure for homogenization.
|
| Growth protocol |
All experiments used Foxo3 littermates or FVB wild-type mice from our own breeding colony. Animals were maintained in a pathogenfree facility administered by U.T. Southwestern Medical Center in accordance with the institutional guidelines of Charles River Laboratories. A maximum of five minutes was allowed to elapse between sacrifice and tissue harvest to prevent RNA degradation and transcriptional changes.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using 1ml of Tripure (Roche) and 20mg of glycogen per manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared (ENZO BioArray IVT kit) following two rounds of RNA amplification from a single pair of ovaries using the Eberwine method of amplification (Phillips and Eberwine, 1996).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 micrograms of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array in the Affymetrix Hybridization oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 per manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Agilent Gene Array Scanner (Affymetrix).
|
| Description |
Gene expression data from PD7 Foxo3 wild-type ovary sample on Affymetrix Mouse Genome 430 2.0 chip.
|
| Data processing |
GCOS v1.4.0.036 with probe sets scaled to a target signal of 250.
|
| |
|
| Submission date |
Jun 21, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Diego H Castrillon |
| E-mail(s) |
diego.castrillon@utsouthwestern.edu
|
| Organization name |
U.T. Southwestern Medical Center
|
| Department |
Pathology
|
| Lab |
Castrillon
|
| Street address |
6000 Harry Hines Blvd. NB6.126
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8249 |
Systematic discovery and classification of ovarian fertility factors. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
