|
| Status |
Public on Aug 02, 2007 |
| Title |
LCM Somatic_3wk. ovary_FVB |
| Sample type |
RNA |
| |
|
| Source name |
Granulosa and stromal cell collected from 3wk. FVB ovary
|
| Organism |
Mus musculus |
| Characteristics |
Somatic cells from 3wk. FVB ovary
|
| Biomaterial provider |
Castrillon Laboratory
|
| Treatment protocol |
Adult mice were sacrificed by CO2 asphyxiation followed by cervical dislocation. Ovaries were dissected and frozen in OCT. Sections were prepared on neutral slides per methods from Arcturus to use in laser capture microdissection.
|
| Growth protocol |
All experiments used Foxo3 littermates or FVB wild-type mice from our own breeding colony. Animals were maintained in a pathogenfree facility administered by U.T. Southwestern Medical Center in accordance with the institutional guidelines of Charles River Laboratories. A maximum of five minutes was allowed to elapse between sacrifice and tissue harvest to prevent RNA degradation and transcriptional changes
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed using Picopure RNA Isolation kit per manufacturer's instructions (Arcturus).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared (ENZO BioArray IVT kit) following two rounds of RNA amplification using the Eberwine method of amplification (Phillips and Eberwine, 1996).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 micrograms of cRNA were hybridized for 16 hr at 45C on Mouse Genome 430 2.0 Array in the Affymetrix Hybridization oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 400 per manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Agilent Gene Array Scanner (Affymetrix).
|
| Description |
Gene expression data on Affymetrix Mouse Genome 430 2.0 chip.
|
| Data processing |
GCOS v1.4.0.036 with probe sets scaled to a target signal of 250.
|
| |
|
| Submission date |
Jun 22, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Diego H Castrillon |
| E-mail(s) |
diego.castrillon@utsouthwestern.edu
|
| Organization name |
U.T. Southwestern Medical Center
|
| Department |
Pathology
|
| Lab |
Castrillon
|
| Street address |
6000 Harry Hines Blvd. NB6.126
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8249 |
Systematic discovery and classification of ovarian fertility factors. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
