|
| Status |
Public on Dec 23, 2007 |
| Title |
Tet integrin alpha7 C2C12 noninduced rep2 |
| Sample type |
RNA |
| |
|
| Source name |
integrin alpha7 C2C12 cells, noninduced
|
| Organism |
Mus musculus |
| Characteristics |
Undifferentiated integrin alpha7 inducible C2C12 cell C2C12 myoblasts, generated using invitrogen T-rex system, passage #7, grown for 36 hours in 5% CO2 humidified incubator before RNA extraction
|
| Treatment protocol |
integrin alpha7 C2C12 cells were cultured at 37 °C for 36 hours in Dulbecco's modified Eagle's medium (DMEM) containing 20% FBS, 0.5% chick embryo extract, 2 mM glutamine, 100 units/ml penicillin, 100 µg/ml streptomycin, and 10 µg/ml kanamycin.
|
| Growth protocol |
integrin alpha7 C2C12 cells were cultured at 37 °C for 36 hours without tetracycline
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions. total RNA were further subject a a cleanup protocol using a Qiagen Rneasy minikit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the manufacturer's instructions (One-Cycle Target Labeling kit, Affymetrix,Inc.)
|
| |
|
| Hybridization protocol |
Following fragmentation, labeled cRNA were hybridized at 45°C for approximately 16 hr to Gene Chip Mouse Genome 430 2.0 Expression Array (Affymetrix). The arrays were stained on Gene Chip Fluidics Workstation 450.
|
| Scan protocol |
Gene Chips were scanned using the affymetrix GeneArray Scanner 3000.
|
| Description |
Gene expression data from undifferentiated integrin alpha7 inducible C2C12 myoblasts incubated in basal media for 36hr without tetracycline
|
| Data processing |
The scanned image was converted into numerical values of the signal intensity (Signal) and into categorical expression level measurement (Absolute Call) using the Affymetrix GCOS V1.1.1 software with default parameters.
|
| |
|
| Submission date |
Jun 27, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jianming Liu |
| E-mail(s) |
liu10@uiuc.edu
|
| Phone |
217-333-6058
|
| Fax |
(217) 244-1648
|
| Organization name |
University of Illinois at urbana-champaign
|
| Department |
Department of Cell and Developmental Biology
|
| Lab |
Dr. Stephen J. Kaufman
|
| Street address |
B107 CLSL, 601 S. Goodwin Avenue
|
| City |
Urbana |
| State/province |
IL |
| ZIP/Postal code |
61801 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8312 |
Enhancing integrin alpha7 expression effect on myoblast transcription |
|
| Relations |
| Reanalyzed by |
GSE119085 |
