|
| Status |
Public on Mar 25, 2008 |
| Title |
WT hippocampus 1/TG hippocampus 4 (LGTC) |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Hippocampus WT mice / Hippocampus DLCK transgenic mice
|
| Organism |
Mus musculus |
| Characteristics |
Male C57/BL6j
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Amplification with Ambion's MessageAmp kit with aa-UTP incorporation and subsequent coupling to GE Healthcare succinimidyl ester-modifed Cy3 and Cy5 dyes
|
| |
|
| Channel 2 |
| Source name |
Hippocampus WT mice / Hippocampus DLCK transgenic mice
|
| Organism |
Mus musculus |
| Characteristics |
Male C57/BL6j
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
Amplification with Ambion's MessageAmp kit with aa-UTP incorporation and subsequent coupling to GE Healthcare succinimidyl ester-modifed Cy3 and Cy5 dyes
|
| |
|
| |
| Hybridization protocol |
Hybridization in SSC/SDS buffer at 55C in GeneTac hybstation
|
| Scan protocol |
Agilent scanner
|
| Description |
none
|
| Data processing |
Loess normalization (limma)
|
| |
|
| Submission date |
Jun 29, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Peter A.C. 't Hoen |
| E-mail(s) |
p.a.c.hoen@lumc.nl
|
| Phone |
+31 71 5269421
|
| Fax |
+31 71 5268285
|
| URL |
http://www.humgen.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Center for Human and Clinical Genetics
|
| Street address |
PO Box 9600
|
| City |
Leiden |
| ZIP/Postal code |
2300 RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5438 |
| Series (1) |
| GSE8349 |
Microarray platform comparison study of hippocampal gene expression in DCLK1 transgenic and wild-type mice |
|
