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Sample GSM206630 Query DataSets for GSM206630
Status Public on Mar 25, 2008
Title WT hippocampus 4/TG hippocampus 2 (LGTC)
Sample type RNA
 
Channel 1
Source name Hippocampus WT mice / Hippocampus DLCK transgenic mice
Organism Mus musculus
Characteristics Male C57/BL6j
Extracted molecule total RNA
Extraction protocol Trizol
Label Cy3
Label protocol Amplification with Ambion's MessageAmp kit with aa-UTP incorporation and subsequent coupling to GE Healthcare succinimidyl ester-modifed Cy3 and Cy5 dyes
 
Channel 2
Source name Hippocampus WT mice / Hippocampus DLCK transgenic mice
Organism Mus musculus
Characteristics Male C57/BL6j
Extracted molecule total RNA
Extraction protocol Trizol
Label Cy5
Label protocol Amplification with Ambion's MessageAmp kit with aa-UTP incorporation and subsequent coupling to GE Healthcare succinimidyl ester-modifed Cy3 and Cy5 dyes
 
 
Hybridization protocol Hybridization in SSC/SDS buffer at 55C in GeneTac hybstation
Scan protocol Agilent scanner
Description none
Data processing Loess normalization (limma)
 
Submission date Jun 29, 2007
Last update date Aug 14, 2011
Contact name Peter A.C. 't Hoen
E-mail(s) p.a.c.hoen@lumc.nl
Phone +31 71 5269421
Fax +31 71 5268285
URL http://www.humgen.nl
Organization name Leiden University Medical Center
Department Center for Human and Clinical Genetics
Street address PO Box 9600
City Leiden
ZIP/Postal code 2300 RC
Country Netherlands
 
Platform ID GPL5438
Series (1)
GSE8349 Microarray platform comparison study of hippocampal gene expression in DCLK1 transgenic and wild-type mice

Data table header descriptions
ID_REF
VALUE Loess normalized log ratios values Cy5 over Cy3 (channel 2 over channel 1)

Data table
ID_REF VALUE
1 -0.0772393537009728
2 -0.0370622950545221
3 -0.0641273812317624
4 -0.0463737921982048
5 -0.0106412093552017
6 -0.222248277693697
7 0.297663499054203
8 0.314272650448195
9 -0.168710416930378
10 0.0969574291599236
11 0.0251146571209457
12 0.0569828469961835
13 -0.0456352696473454
14 -0.00979491174048619
15 0.131256539128412
16 -0.0698906709676763
17 -0.184471342182553
18 -0.157077331379623
19 -0.0674763998959282
20 -0.121632040117812

Total number of rows: 23232

Table truncated, full table size 555 Kbytes.




Supplementary file Size Download File type/resource
GSM206630.gpr.gz 1.8 Mb (ftp)(http) GPR
Processed data included within Sample table

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