|
| Status |
Public on Dec 31, 2007 |
| Title |
Expression data from Cas exon 2-hetero deficient primary fibroblast2 |
| Sample type |
RNA |
| |
|
| Source name |
MEF36 hetero
|
| Organism |
Mus musculus |
| Characteristics |
Strain: B6, mouse embryonal fibroblasts, Cas exon 2+/∆,MEF36 hetero
|
| Treatment protocol |
Embryos at 12.5 dpc were collected and primary embryonic fibroblasts were isolated from them.
|
| Growth protocol |
Cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal calf serum (FCS), penicillin and streptomycin at 37 degree with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degree on GeneChip Mouse Genome 430 2.0 Array . GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Cas exon 2+/∆ mice were intercrossed and embryos at 12.5 dpc were collected. Heads and inner organ tissues were used for genotyping and primary embryonic fibroblasts were isolated from the remaining of embryos and cultured
|
| Data processing |
The data were analyzed using GeneSpring (Agilent Technologies).
|
| |
|
| Submission date |
Jul 03, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Eiso Hiyama |
| E-mail(s) |
eiso@hiroshima-u.ac.jp
|
| Phone |
81822575951
|
| Organization name |
Hiroshima University
|
| Department |
Natural Science Center for Basic Research and Deve
|
| Lab |
LIfe Science
|
| Street address |
1-2-3, Kasumi, Minami-ku
|
| City |
Hiroshima |
| State/province |
Hiroshima |
| ZIP/Postal code |
734-8551 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8357 |
Functional analysis of exon 2 of p130Cas in fibroblasts derived from exon 2-specific knockout mice. |
|
| Relations |
| Reanalyzed by |
GSE119085 |
