|
| Status |
Public on Jul 06, 2007 |
| Title |
CET at 150 µg/kg BW for 24 h + rhLH for 8h_3 |
| Sample type |
RNA |
| |
|
| Source name |
CL from female bonnet monkey (CET at 150 µg/kg BW for 24 h + rhLH for 8h)
|
| Organism |
Macaca radiata |
| Characteristics |
CL from female bonnet monkey (CET at 150 µg/kg BW for 24 h + rhLH for 8h)
|
| Biomaterial provider |
R. Medhamurthy
|
| Treatment protocol |
Female monkeys receiving CET treatment on day 7 of luteal phase were administered 20 IU/kg BW of rhLH or PBS (0.5 ml) i.v. at 24 h post CET treatment and blood samples were collected before and after rhLH/PBS treatments. To study changes in gene expression associated with LH replacement following inhibition of endogenous LH secretion by CET, CL was collected at 8 h (n=2) following LH replacement from female monkeys treated previously with CET for 24 h. Monkeys receiving CET on day 7 of luteal phase and 1X PBS at 24 h following CET treatment served as control (n=2) for the present study. Corpora lutea following different treatments were cut into small quarters and snap frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol reagent (Sigma Aldrich Co., St.Louis, MO) followed by clean up step using RNeasy mini purification kit (# 74104, Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001)
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001)
|
| Scan protocol |
Scan protocol Standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001) using scanner 3000 series
|
| Description |
Description Probe level measurements produced by Affymetrix microarray suite 5.0. Analysis performed at level of raw signals (See Affymetrix Microarray suite 5.0 reference on web site for description).
|
| Data processing |
Data processing MAS5.0, data was normalized with standard Affymetrix protocols (Chip Expression Analysis Technical Manual rev.3, 2001)
|
| |
|
| Submission date |
Jul 04, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
priyanka samji |
| E-mail(s) |
priyanka@mrdg.iisc.ernet.in
|
| Phone |
91-80-22932490
|
| Fax |
91-80-23600999
|
| Organization name |
Indian Institute of Science
|
| Department |
Molecular Reproduction Development and Genetics
|
| Lab |
R. Medhamurthy's lab
|
| Street address |
Malleswaram
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560012 |
| Country |
India |
| |
|
| Platform ID |
GPL3535 |
| Series (1) |
| GSE8371 |
Effect of LH replacement on luteal transcriptome following CET-induced luteolysis |
|
