|
| Status |
Public on Aug 01, 2007 |
| Title |
Adult murine BM, Lin-Sca1-Kit+Itga2b-FcgRlowSlamf1-Englow 3 |
| Sample type |
RNA |
| |
|
| Source name |
Adult murine BM from 10-14 weeks old mice
|
| Organism |
Mus musculus |
| Characteristics |
Wild type C57bl/6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard Affymetrix(TM); GeneChip Expression Analysis Technical Manual
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
|
| Description |
Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
|
| Data processing |
Expression values were calculated by RMA express (http://rmaexpress.bmbolstad.com/) using background adjustment and quantile normalization.
|
| |
|
| Submission date |
Jul 05, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Robert Månsson |
| E-mail(s) |
robert.mansson@ki.se
|
| Organization name |
Lund University
|
| Department |
Hemapotoietic stem cell biology
|
| Street address |
Klinikgatan 26, BMC B12
|
| City |
Lund |
| ZIP/Postal code |
221 84 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8407 |
Elucidation of the phenotypic, functional and molecular topography of a myeloerythroid progenitor cell hierarchy |
|
| Relations |
| Reanalyzed by |
GSE55732 |
| Reanalyzed by |
GSE56952 |
| Reanalyzed by |
GSE119085 |
