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Sample GSM207682 Query DataSets for GSM207682
Status Public on Aug 01, 2007
Title Adult murine BM, Lin-Sca1-Kit+Itga2b-FcgRlowSlamf1+Englow 1
Sample type RNA
 
Source name Adult murine BM from 10-14 weeks old mice
Organism Mus musculus
Characteristics Wild type C57bl/6
Extracted molecule total RNA
Extraction protocol Cells were directly sorted into buffer RLT and RNA prepared using RNeasy Micro (Qiagen) according to manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix(TM); GeneChip Expression Analysis Technical Manual
 
Hybridization protocol Following fragmentation, 10 microg of cRNA was hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Chips were scanned using an Affymetrix GeneChip Scanner 3000. Chip target intensity was arbitrarily scaled to 100 before rendering CHP files.
Description Gene expression data from LSKCD34-Flt3- cells FACS sorted from adult BM
Data processing Expression values were calculated by RMA express (http://rmaexpress.bmbolstad.com/) using background adjustment and quantile normalization.
 
Submission date Jul 05, 2007
Last update date Aug 28, 2018
Contact name Robert MÃ¥nsson
E-mail(s) robert.mansson@ki.se
Organization name Lund University
Department Hemapotoietic stem cell biology
Street address Klinikgatan 26, BMC B12
City Lund
ZIP/Postal code 221 84
Country Sweden
 
Platform ID GPL1261
Series (1)
GSE8407 Elucidation of the phenotypic, functional and molecular topography of a myeloerythroid progenitor cell hierarchy
Relations
Reanalyzed by GSE55732
Reanalyzed by GSE56952
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE RMA Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1415670_at 296.27396 P
1415671_at 208.300632 P
1415672_at 941.723452 P
1415673_at 144.58262 P
1415674_a_at 196.903046 P
1415675_at 159.453711 P
1415676_a_at 769.807507 P
1415677_at 76.920328 P
1415678_at 835.352526 P
1415679_at 683.939626 P
1415680_at 391.571903 P
1415681_at 208.787509 P
1415682_at 53.259132 P
1415683_at 906.111156 P
1415684_at 71.229158 P
1415685_at 272.91851 P
1415686_at 377.280591 P
1415687_a_at 155.004649 P
1415688_at 788.922656 P
1415689_s_at 175.745057 P

Total number of rows: 45101

Table truncated, full table size 1024 Kbytes.




Supplementary file Size Download File type/resource
GSM207682.CEL.gz 5.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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