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Sample GSM209538 Query DataSets for GSM209538
Status Public on Jul 11, 2007
Title Control MO, 10s, rep2
Sample type RNA
 
Source name zebrafish 10 somite stage embryos
Organism Danio rerio
Characteristics AB background, 10 somite embryos
Treatment protocol Embryos were injected at the 1-2 cell stage with control (pbx2 mutant) morpholinos
Extracted molecule total RNA
Extraction protocol Trizol extraction and Qiagen clean up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol cRNA was hybridized onto Affymetrix zebrafish genome arrays
Scan protocol GeneChips were scanned with GENECHIP software
Description Gene expression data from zebrafish 10 somite stage embryos
Data processing The data were normalized by RMA using the Affy package of Bioconductor
 
Submission date Jul 10, 2007
Last update date Aug 14, 2011
Contact name Yi Cao
E-mail(s) ycao@fhcrc.org
Phone 206-667-5278
Organization name Fred Hutchinson CRC
Department Human Biology
Lab Tapscott
Street address 1100 Fairview Ave. N
City Seatttle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL1319
Series (1)
GSE8428 The expression profiles of control embryos and pbx2-MO;pbx4-MO embryos at 10 somites and at 18 somites.

Data table header descriptions
ID_REF Affy ID
VALUE RMA calculated signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 7.134
AFFX-BioB-5_at 8.06
AFFX-BioB-M_at 7.688
AFFX-BioC-3_at 8.747
AFFX-BioC-5_at 8.74
AFFX-BioDn-3_at 11.131
AFFX-BioDn-5_at 10.057
AFFX-CreX-3_at 13.196
AFFX-CreX-5_at 12.419
AFFX-DapX-3_at 9.57
AFFX-DapX-5_at 7.886
AFFX-DapX-M_at 9.144
AFFX-Dr-AB076373-1_at 5.541
AFFX-Dr-acta1-3_at 11.229
AFFX-Dr-acta1-5_at 8.615
AFFX-Dr-acta1-5_x_at 10.963
AFFX-Dr-acta1-M_at 10.455
AFFX-Dr-AF292559-1_at 4.001
AFFX-Dr-AF292559-2_s_at 3.225
AFFX-Dr-AF292559-3_s_at 3.594

Total number of rows: 15617

Table truncated, full table size 347 Kbytes.




Supplementary file Size Download File type/resource
GSM209538.CEL.gz 2.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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