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Sample GSM209541 Query DataSets for GSM209541
Status Public on Jul 11, 2007
Title pbx2-MO;pbx4-MO, 10s, rep2
Sample type RNA
 
Source name zebrafish 10 somite stage embryos
Organism Danio rerio
Characteristics AB background, 10 somite embryos
Treatment protocol Embryos were injected at the 1-2 cell stage with pbx2 and pbx4 morpholinos
Extracted molecule total RNA
Extraction protocol Trizol extraction and Qiagen clean up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol cRNA was hybridized onto Affymetrix zebrafish genome arrays
Scan protocol GeneChips were scanned with GENECHIP software
Description Gene expression data from zebrafish 10 somite stage embryos
Data processing The data were normalized by RMA using the Affy package of Bioconductor
 
Submission date Jul 10, 2007
Last update date Aug 14, 2011
Contact name Yi Cao
E-mail(s) ycao@fhcrc.org
Phone 206-667-5278
Organization name Fred Hutchinson CRC
Department Human Biology
Lab Tapscott
Street address 1100 Fairview Ave. N
City Seatttle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL1319
Series (1)
GSE8428 The expression profiles of control embryos and pbx2-MO;pbx4-MO embryos at 10 somites and at 18 somites.

Data table header descriptions
ID_REF Affy ID
VALUE RMA calculated signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 6.971
AFFX-BioB-5_at 8.021
AFFX-BioB-M_at 7.563
AFFX-BioC-3_at 8.582
AFFX-BioC-5_at 8.645
AFFX-BioDn-3_at 11.057
AFFX-BioDn-5_at 9.989
AFFX-CreX-3_at 13.14
AFFX-CreX-5_at 12.42
AFFX-DapX-3_at 9.704
AFFX-DapX-5_at 8.054
AFFX-DapX-M_at 9.318
AFFX-Dr-AB076373-1_at 5.634
AFFX-Dr-acta1-3_at 11.162
AFFX-Dr-acta1-5_at 8.058
AFFX-Dr-acta1-5_x_at 10.856
AFFX-Dr-acta1-M_at 10.429
AFFX-Dr-AF292559-1_at 4.002
AFFX-Dr-AF292559-2_s_at 3.33
AFFX-Dr-AF292559-3_s_at 3.53

Total number of rows: 15617

Table truncated, full table size 347 Kbytes.




Supplementary file Size Download File type/resource
GSM209541.CEL.gz 2.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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