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Sample GSM209543 Query DataSets for GSM209543
Status Public on Jul 11, 2007
Title Control MO, 18s, rep1
Sample type RNA
Source name zebrafish 18 somite stage embryos
Organism Danio rerio
Characteristics AB background, 18 somite embryos
Treatment protocol Embryos were injected at the 1-2 cell stage with control (pbx2 mutant) morpholinos
Extracted molecule total RNA
Extraction protocol Trizol extraction and Qiagen clean up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
Hybridization protocol cRNA was hybridized onto Affymetrix zebrafish genome arrays
Scan protocol GeneChips were scanned with GENECHIP software
Description Gene expression data from zebrafish 18 somite stage embryos
Data processing The data were normalized by RMA using the Affy package of Bioconductor
Submission date Jul 10, 2007
Last update date Aug 14, 2011
Contact name Yi Cao
Phone 206-667-5278
Organization name Fred Hutchinson CRC
Department Human Biology
Lab Tapscott
Street address 1100 Fairview Ave. N
City Seatttle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL1319
Series (1)
GSE8428 The expression profiles of control embryos and pbx2-MO;pbx4-MO embryos at 10 somites and at 18 somites.

Data table header descriptions
VALUE RMA calculated signal intensity

Data table
AFFX-BioB-3_at 6.128
AFFX-BioB-5_at 7.244
AFFX-BioB-M_at 6.941
AFFX-BioC-3_at 7.959
AFFX-BioC-5_at 7.773
AFFX-BioDn-3_at 10.187
AFFX-BioDn-5_at 9.237
AFFX-CreX-3_at 12.37
AFFX-CreX-5_at 11.431
AFFX-DapX-3_at 8.176
AFFX-DapX-5_at 6.613
AFFX-DapX-M_at 8.051
AFFX-Dr-AB076373-1_at 5.016
AFFX-Dr-acta1-3_at 12.262
AFFX-Dr-acta1-5_at 9.763
AFFX-Dr-acta1-5_x_at 11.618
AFFX-Dr-acta1-M_at 11.384
AFFX-Dr-AF292559-1_at 3.303
AFFX-Dr-AF292559-2_s_at 3.021
AFFX-Dr-AF292559-3_s_at 3.206

Total number of rows: 15617

Table truncated, full table size 346 Kbytes.

Supplementary file Size Download File type/resource
GSM209543.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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