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Sample GSM209547 Query DataSets for GSM209547
Status Public on Jul 11, 2007
Title pbx2-MO;pbx4-MO, 18s, rep2
Sample type RNA
 
Source name zebrafish 18 somite stage embryos
Organism Danio rerio
Characteristics AB background, 18 somite embryos
Treatment protocol Embryos were injected at the 1-2 cell stage with pbx2 and pbx4 morpholinos
Extracted molecule total RNA
Extraction protocol Trizol extraction and Qiagen clean up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
 
Hybridization protocol cRNA was hybridized onto Affymetrix zebrafish genome arrays
Scan protocol GeneChips were scanned with GENECHIP software
Description Gene expression data from zebrafish 18 somite stage embryos
Data processing The data were normalized by RMA using the Affy package of Bioconductor
 
Submission date Jul 10, 2007
Last update date Aug 14, 2011
Contact name Yi Cao
E-mail(s) ycao@fhcrc.org
Phone 206-667-5278
Organization name Fred Hutchinson CRC
Department Human Biology
Lab Tapscott
Street address 1100 Fairview Ave. N
City Seatttle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL1319
Series (1)
GSE8428 The expression profiles of control embryos and pbx2-MO;pbx4-MO embryos at 10 somites and at 18 somites.

Data table header descriptions
ID_REF Affy ID
VALUE RMA calculated signal intensity

Data table
ID_REF VALUE
AFFX-BioB-3_at 6.321
AFFX-BioB-5_at 7.159
AFFX-BioB-M_at 7.012
AFFX-BioC-3_at 8.095
AFFX-BioC-5_at 7.769
AFFX-BioDn-3_at 10.286
AFFX-BioDn-5_at 9.39
AFFX-CreX-3_at 12.68
AFFX-CreX-5_at 11.651
AFFX-DapX-3_at 8.301
AFFX-DapX-5_at 6.751
AFFX-DapX-M_at 8.099
AFFX-Dr-AB076373-1_at 4.843
AFFX-Dr-acta1-3_at 11.604
AFFX-Dr-acta1-5_at 8.63
AFFX-Dr-acta1-5_x_at 10.875
AFFX-Dr-acta1-M_at 10.991
AFFX-Dr-AF292559-1_at 3.347
AFFX-Dr-AF292559-2_s_at 2.871
AFFX-Dr-AF292559-3_s_at 3.127

Total number of rows: 15617

Table truncated, full table size 346 Kbytes.




Supplementary file Size Download File type/resource
GSM209547.CEL.gz 2.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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