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Sample GSM209548 Query DataSets for GSM209548
Status Public on Jul 11, 2007
Title pbx2-MO;pbx4-MO, 18s, rep3
Sample type RNA
Source name zebrafish 18 somite stage embryos
Organism Danio rerio
Characteristics AB background, 18 somite embryos
Treatment protocol Embryos were injected at the 1-2 cell stage with pbx2 and pbx4 morpholinos
Extracted molecule total RNA
Extraction protocol Trizol extraction and Qiagen clean up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA.
Hybridization protocol cRNA was hybridized onto Affymetrix zebrafish genome arrays
Scan protocol GeneChips were scanned with GENECHIP software
Description Gene expression data from zebrafish 18 somite stage embryos
Data processing The data were normalized by RMA using the Affy package of Bioconductor
Submission date Jul 10, 2007
Last update date Aug 14, 2011
Contact name Yi Cao
Phone 206-667-5278
Organization name Fred Hutchinson CRC
Department Human Biology
Lab Tapscott
Street address 1100 Fairview Ave. N
City Seatttle
State/province WA
ZIP/Postal code 98109
Country USA
Platform ID GPL1319
Series (1)
GSE8428 The expression profiles of control embryos and pbx2-MO;pbx4-MO embryos at 10 somites and at 18 somites.

Data table header descriptions
VALUE RMA calculated signal intensity

Data table
AFFX-BioB-3_at 6.118
AFFX-BioB-5_at 7.355
AFFX-BioB-M_at 6.979
AFFX-BioC-3_at 7.985
AFFX-BioC-5_at 7.736
AFFX-BioDn-3_at 10.001
AFFX-BioDn-5_at 9.15
AFFX-CreX-3_at 12.191
AFFX-CreX-5_at 11.47
AFFX-DapX-3_at 8.124
AFFX-DapX-5_at 6.499
AFFX-DapX-M_at 7.951
AFFX-Dr-AB076373-1_at 4.748
AFFX-Dr-acta1-3_at 12.114
AFFX-Dr-acta1-5_at 8.864
AFFX-Dr-acta1-5_x_at 11.166
AFFX-Dr-acta1-M_at 11.39
AFFX-Dr-AF292559-1_at 3.405
AFFX-Dr-AF292559-2_s_at 2.935
AFFX-Dr-AF292559-3_s_at 3.123

Total number of rows: 15617

Table truncated, full table size 346 Kbytes.

Supplementary file Size Download File type/resource
GSM209548.CEL.gz 2.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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