|
| Status |
Public on Dec 18, 2007 |
| Title |
5,F9 ko cells |
| Sample type |
RNA |
| |
|
| Source name |
F9 RAR gamma knock out cells
|
| Organism |
Mus musculus |
| Characteristics |
Retinoic acid receptor (RAR) gamma F9 cells from mouse
|
| Treatment protocol |
Retinoic Acid 1uM at dim light or vehicle control ethanol
|
| Growth protocol |
5% FCS +5%CS in DMEM medium at 10% CO2, 37°C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA according to the protocol
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the standard protocol from 15 ug total RNA (Expression technical manual 2005)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized for 16 hr at 45 C on gene chip MG-430.2
|
| Scan protocol |
Gene chips were scanned using the hewlett-packard Gene Array Scanner G2500A
|
| Description |
comparison between F9 wild type and F9 RAR gamma knock out cells treated with retinoic acid or ethanol in mouse to look at differentially expressed genes
|
| Data processing |
Microarray Suite version 5.0 (MAS5.0) using default analysis settings and global scaling as normalized method.
|
| |
|
| Submission date |
Jul 10, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Dan Su |
| E-mail(s) |
das2015@med.cornell.edu
|
| Phone |
212-746-6262
|
| Organization name |
Weill Cornell Medical College
|
| Department |
Pharmacology
|
| Lab |
Lorrain J Gudas
|
| Street address |
1300 york Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10021 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE8431 |
Microarray screening of RARgamma responsive genes in F9 teratocarcinoma cells |
|
| Relations |
| Reanalyzed by |
GSE119085 |
