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Sample GSM2096882 Query DataSets for GSM2096882
Status Public on Nov 23, 2017
Title RAW-264.7 empty vector, clone 4
Sample type RNA
 
Source name RAW-264.7 macrophage/monocyte cell line
Organism Mus musculus
Characteristics cell line: RAW-264.7
transfection: pEF6/V5-His TOPO vector
Treatment protocol No additional treatments
Growth protocol RAW-264.7 stable transfectants were propagated in DMEM+Glutamax medium (ThermoFisher Scientific) supplemented with 10% fetal bovine serum, 1% pen/strep and 6 µg/ml blasticidin S
Extracted molecule total RNA
Extraction protocol Isolation of total RNA from cells was performed using E.Z.N.A. total RNA Kit I (Omega bio-tek) according to manufacturer’s recommendation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Data processing The data were analyzed with a commercial software called JMP Genomics, version 5, from SAS. Gene expression profiling was performed using arrays of human mouse430_2-type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
 
Submission date Mar 23, 2016
Last update date Nov 23, 2017
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE79521 Expression data from RAW-264.7 cells transfected with empty vector and mouse FoxQ1 expression construct

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100009600_at 7.092973259
100012_at 6.102946883
100017_at 8.969530112
100019_at 8.469303052
100034251_at 11.45959761
100036521_at 9.407362088
100037258_at 8.273897093
100037278_at 7.995734898
100038570_at 7.239417361
100038635_at 6.475343089
100038680_at 6.74195832
100038887_at 11.93108118
100038959_at 8.022177881
100039026_at 8.128614609
100039027_at 6.251181082
100039094_at 7.298913677
100039235_at 7.734125971
100039282_at 5.952500921
100039284_at 7.375673232
100039307_at 6.999566657

Total number of rows: 16556

Table truncated, full table size 343 Kbytes.




Supplementary file Size Download File type/resource
GSM2096882_Kzhyshkowska_081210_empty_Vector_Klon_4_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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