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Sample GSM2096883 Query DataSets for GSM2096883
Status Public on Nov 23, 2017
Title RAW-264.7 empty vector, clone 5
Sample type RNA
 
Source name RAW-264.7 macrophage/monocyte cell line
Organism Mus musculus
Characteristics cell line: RAW-264.7
transfection: pEF6/V5-His TOPO vector
Treatment protocol No additional treatments
Growth protocol RAW-264.7 stable transfectants were propagated in DMEM+Glutamax medium (ThermoFisher Scientific) supplemented with 10% fetal bovine serum, 1% pen/strep and 6 µg/ml blasticidin S
Extracted molecule total RNA
Extraction protocol Isolation of total RNA from cells was performed using E.Z.N.A. total RNA Kit I (Omega bio-tek) according to manufacturer’s recommendation
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
 
Hybridization protocol Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
Scan protocol Affymetrix GeneArray Scanner3000
Data processing The data were analyzed with a commercial software called JMP Genomics, version 5, from SAS. Gene expression profiling was performed using arrays of human mouse430_2-type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
 
Submission date Mar 23, 2016
Last update date Nov 23, 2017
Contact name Carsten Sticht
Organization name University Heidelberg
Department ZMF
Street address Theodor-Kutzer-Ufer
City Mannheim
ZIP/Postal code 68169
Country Germany
 
Platform ID GPL14661
Series (1)
GSE79521 Expression data from RAW-264.7 cells transfected with empty vector and mouse FoxQ1 expression construct

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
100009600_at 7.12946654
100012_at 6.167641836
100017_at 9.069448496
100019_at 8.738025445
100034251_at 11.72854636
100036521_at 9.047403569
100037258_at 7.961619514
100037278_at 8.247565465
100038570_at 7.293399983
100038635_at 6.392113847
100038680_at 6.688496017
100038887_at 12.17973153
100038959_at 8.310851728
100039026_at 7.94970807
100039027_at 6.224663752
100039094_at 7.280861677
100039235_at 7.53461144
100039282_at 5.901083754
100039284_at 7.312907888
100039307_at 6.879896009

Total number of rows: 16556

Table truncated, full table size 343 Kbytes.




Supplementary file Size Download File type/resource
GSM2096883_Kzhyshkowska_081210_empty_Vector_Klon_5_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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