|
| Status |
Public on Nov 23, 2017 |
| Title |
RAW-264.7 FoxQ1, clone 7.2 |
| Sample type |
RNA |
| |
|
| Source name |
RAW-264.7 macrophage/monocyte cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: RAW-264.7
transfection: mouse FoxQ1 expression construct
|
| Treatment protocol |
No additional treatments
|
| Growth protocol |
RAW-264.7 stable transfectants were propagated in DMEM+Glutamax medium (ThermoFisher Scientific) supplemented with 10% fetal bovine serum, 1% pen/strep and 6 µg/ml blasticidin S
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Isolation of total RNA from cells was performed using E.Z.N.A. total RNA Kit I (Omega bio-tek) according to manufacturer’s recommendation
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 5, from SAS. Gene expression profiling was performed using arrays of human mouse430_2-type from Affymetrix. A Custom CDF Version 13 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Mar 23, 2016 |
| Last update date |
Nov 23, 2017 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL14661 |
| Series (1) |
| GSE79521 |
Expression data from RAW-264.7 cells transfected with empty vector and mouse FoxQ1 expression construct |
|
